2026-04-29T12:24:08Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/52262024-09-27T08:19:28Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Evrard, Solene M. author Lecce, Laura author Michelis, Katherine C. author Nomura-Kitabayashi, Aya author Pandey, Gaurav author Purushothaman, K-Raman author d'Escamard, Valentina author Li, Jennifer R. author Hadri, Lahouaria author Fujitani, Kenji author Moreno, Pedro R. author Benard, Ludovic author Rimmele, Pauline author Cohain, Ariella author Mecham, Brigham author Randolph, Gwendalyn J. author Nabel, Elizabeth G. author Hajjar, Roger author Fuster, Valentin author Boehm, Manfred author Kovacic, Jason C. author 2016 Endothelial to mesenchymal transition (EndMT) plays a major role during development, and also contributes to several adult cardiovascular diseases. Importantly, mesenchymal cells including fibroblasts are prominent in atherosclerosis, with key functions including regulation of: inflammation, matrix and collagen production, and plaque structural integrity. However, little is known about the origins of atherosclerosis-associated fibroblasts. Here we show using endothelial-specific lineage-tracking that EndMT-derived fibroblast-like cells are common in atherosclerotic lesions, with EndMT-derived cells expressing a range of fibroblast-specific markers. In vitro modelling confirms that EndMT is driven by TGF-beta signalling, oxidative stress and hypoxia; all hallmarks of atherosclerosis. `Transitioning' cells are readily detected in human plaques co-expressing endothelial and fibroblast/mesenchymal proteins, indicative of EndMT. The extent of EndMT correlates with an unstable plaque phenotype, which appears driven by altered collagen-MMP production in EndMT-derived cells. We conclude that EndMT contributes to atherosclerotic patho-biology and is associated with complex plaques that may be related to clinical events. Nat Commun. 2016; 7:11853 10.1038/ncomms11853 2041-1723 Nature Communications 27340017 http://hdl.handle.net/20.500.12105/5226 FIBROBLAST ACTIVATION PROTEIN AMERICAN-HEART-ASSOCIATION MARROW-DERIVED CELLS SMOOTH-MUSCLE-CELLS IN-VIVO PULMONARY-HYPERTENSION CARDIAC FIBROSIS RENAL FIBROSIS EXPRESSION MICE Endothelial to mesenchymal transition is common in atherosclerotic lesions and is associated with plaque instability