2026-08-29T09:25:23Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/52222024-10-31T11:41:00Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
Sala-Vila, Aleix
author
Navarro-Lerida, Inmaculada
author
Sanchez-Alvarez, Miguel
author
Bosch, Marta
author
Calvo, Carlos
author
Lopez, Juan Antonio
author
Calvo, Enrique
author
Ferguson, Charles
author
Giacomello, Marta
author
Serafini, Annalisa
author
Scorrano, Luca
author
Enriquez, Jose Antonio
author
Balsinde, Jesus
author
Parton, Robert G.
author
Vazquez, Jesus
author
Pol, Albert
author
del Pozo, Miguel Angel
funder
Ministerio de Economía y Competitividad (España)
funder
Fundación La Marató TV3
funder
Worldwide Cancer Research
funder
Asociación Española Contra el Cáncer
funder
Fundación ProCNIC
funder
Institució Catalana de Recerca i Estudis Avançats
2017-10-30T13:15:44Z
2017-10-30T13:15:44Z
2016
Sci Rep. 2016; 6:27351
10.1038/srep27351
2045-2322
Scientific Reports
27272971
http://hdl.handle.net/20.500.12105/5222
The mitochondria-associated membrane (MAM) is a specialized subdomain of the endoplasmic reticulum (ER) which acts as an intracellular signaling hub. MAM dysfunction has been related to liver disease. We report a high-throughput mass spectrometry-based proteomics characterization of MAMs from mouse liver, which portrays them as an extremely complex compartment involved in different metabolic processes, including steroid metabolism. Interestingly, we identified caveolin-1 (CAV1) as an integral component of hepatic MAMs, which determine the relative cholesterol content of these ER subdomains. Finally, a detailed comparative proteomics analysis between MAMs from wild type and CAV1-deficient mice suggests that functional CAV1 contributes to the recruitment and regulation of intracellular steroid and lipoprotein metabolism-related processes accrued at MAMs. The potential impact of these novel aspects of CAV1 biology on global cell homeostasis and disease is discussed.
eng
ER MEMBRANES
ALZHEIMER-DISEASE
CELLS
CHOLESTEROL
DYSFUNCTION
PROTEIN-1
DROPLETS
Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice
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