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oai:repisalud.isciii.es:20.500.12105/52222024-10-31T11:41:00Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607

00925njm 22002777a 4500 dc Sala-Vila, Aleix author Navarro-Lerida, Inmaculada author Sanchez-Alvarez, Miguel author Bosch, Marta author Calvo, Carlos author Lopez, Juan Antonio author Calvo, Enrique author Ferguson, Charles author Giacomello, Marta author Serafini, Annalisa author Scorrano, Luca author Enriquez, Jose Antonio author Balsinde, Jesus author Parton, Robert G. author Vazquez, Jesus author Pol, Albert author del Pozo, Miguel Angel author 2016 The mitochondria-associated membrane (MAM) is a specialized subdomain of the endoplasmic reticulum (ER) which acts as an intracellular signaling hub. MAM dysfunction has been related to liver disease. We report a high-throughput mass spectrometry-based proteomics characterization of MAMs from mouse liver, which portrays them as an extremely complex compartment involved in different metabolic processes, including steroid metabolism. Interestingly, we identified caveolin-1 (CAV1) as an integral component of hepatic MAMs, which determine the relative cholesterol content of these ER subdomains. Finally, a detailed comparative proteomics analysis between MAMs from wild type and CAV1-deficient mice suggests that functional CAV1 contributes to the recruitment and regulation of intracellular steroid and lipoprotein metabolism-related processes accrued at MAMs. The potential impact of these novel aspects of CAV1 biology on global cell homeostasis and disease is discussed. Sci Rep. 2016; 6:27351 10.1038/srep27351 2045-2322 Scientific Reports 27272971 http://hdl.handle.net/20.500.12105/5222 ER MEMBRANES ALZHEIMER-DISEASE CELLS CHOLESTEROL DYSFUNCTION PROTEIN-1 DROPLETS Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice