2026-06-30T02:01:10Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/52112024-09-27T08:29:11Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607

00925njm 22002777a 4500 dc del Olmo, Ivan author Lopez, Juan Antonio author Vazquez, Jesus author Raynaud, Cecile author Pineiro, Manuel author Jarillo, Jose A. author 2016 Arabidopsis ESD7 locus encodes the catalytic subunit of the DNA Pol I mu involved in the synthesis of the DNA leading strand and is essential for embryo viability. The hypomorphic allele esd7-1 is viable but displays a number of pleiotropic phenotypic alterations including an acceleration of flowering time. Furthermore, Pol I mu is involved in the epigenetic silencing of the floral integrator genes FT and SOC1, but the molecular nature of the transcriptional gene silencing mechanisms involved remains elusive. Here we reveal that ESD7 interacts with components of the PRC2 such as CLF, EMF2 and MSI1, and that mutations in ESD7 cause a decrease in the levels of the H3K27me3 mark present in the chromatin of FT and SOC1. We also demonstrate that a domain of the C-terminal region of ESD7 mediates the binding to the different PRC2 components and this interaction is necessary for the proper recruitment of PRC2 to FT and SOC1 chromatin. We unveil the existence of interplay between the DNA replication machinery and the PcG complexes in epigenetic transcriptional silencing. These observations provide an insight into the mechanisms ensuring that the epigenetic code at pivotal loci in developmental control is faithfully transmitted to the progeny of eukaryotic cells. Nucleic Acids Res. 2016; 44(12):5597-614 10.1093/nar/gkw156 1362-4962 0305-1048 Nucleic Acids Research 26980282 http://hdl.handle.net/20.500.12105/5211 FLOWERING-LOCUS-T CHROMATIN ASSEMBLY FACTOR-1 HISTONE H3 HETEROCHROMATIN PROTEIN-1 CATALYTIC SUBUNIT SHADOW DOMAIN SALT STRESS CELL-CYCLE REPLICATION THALIANA Arabidopsis DNA polymerase epsilon recruits components of Polycomb repressor complex to mediate epigenetic gene silencing