2026-05-22T01:55:54Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/52062024-09-27T07:55:39Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Captur, Gabriella
author
Wilson, Robert
author
Bennett, Michael F.
author
Luxan, Guillermo
author
Nasis, Arthur
author
de la Pompa, Jose Luis
author
Moon, James C.
author
Mohun, Timothy J.
funder
Medical Research Council (Reino Unido)
funder
Higher Education Funding Council for England
funder
Ministerio de Economía y Competitividad (España)
funder
National Institute for Health Research (Reino Unido)
2017-10-30T13:15:40Z
2017-10-30T13:15:40Z
2016
J Anat. 2016; 229(2):314-25
10.1111/joa.12465
1469-7580
0021-8782
Journal of Anatomy
27020702
http://hdl.handle.net/20.500.12105/5206
Formation of trabeculae in the embryonic heart and the remodelling that occurs prior to birth is a conspicuous, but poorly understood, feature of vertebrate cardiogenesis. Mutations disrupting trabecular development in the mouse are frequently embryonic lethal, testifying to the importance of the trabeculae, and aberrant trabecular structure is associated with several human cardiac pathologies. Here, trabecular architecture in the developing mouse embryo has been analysed using high-resolution episcopic microscopy (HREM) and three-dimensional (3D) modelling. This study shows that at all stages from mid-gestation to birth, the ventricular trabeculae comprise a complex meshwork of myocardial strands. Such an arrangement defies conventional methods of measurement, and an approach based upon fractal algorithms has been used to provide an objective measure of trabecular complexity. The extent of trabeculation as it changes along the length of left and right ventricles has been quantified, and the changes that occur from formation of the four-chambered heart until shortly before birth have been mapped. This approach not only measures qualitative features evident from visual inspection of 3D models, but also detects subtle, consistent and regionally localised differences that distinguish each ventricle and its developmental stage. Finally, the combination of HREM imaging and fractal analysis has been applied to analyse changes in embryonic heart structure in a genetic mouse model in which trabeculation is deranged. It is shown that myocardial deletion of the Notch pathway component Mib1 (Mib1(flox/flox); cTnT-cre) results in a complex array of abnormalities affecting trabeculae and other parts of the heart.
eng
Cardiac embryology
Cardiogenesis
Developmental biology
Non-compaction cardiomyopathy
VENTRICULAR NONCOMPACTION CARDIOMYOPATHY
RESOLUTION EPISCOPIC MICROSCOPY
CONDUCTION SYSTEM
HEART DEVELOPMENT
NON-COMPACTION
PROLIFERATION
ARCHITECTURE
EXPRESSION
MUTATIONS
Morphogenesis of myocardial trabeculae in the mouse embryo
journal article
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https://repisalud.isciii.es/bitstreams/35ffb27d-b8b7-49df-bd62-b9a1de42b708/download
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af64989cdeac7897db277119766831ef
2511040
application/pdf
MorphogenesisOfMyocardialTrabeculaeInTheMouse_2016
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d7b3927f1967e3eff81ccd9c098d9584
51715
text/plain
MorphogenesisOfMyocardialTrabeculaeInTheMouse_2016.txt