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oai:repisalud.isciii.es:20.500.12105/52062024-09-27T07:55:39Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Captur, Gabriella author Wilson, Robert author Bennett, Michael F. author Luxan, Guillermo author Nasis, Arthur author de la Pompa, Jose Luis author Moon, James C. author Mohun, Timothy J. author 2016 Formation of trabeculae in the embryonic heart and the remodelling that occurs prior to birth is a conspicuous, but poorly understood, feature of vertebrate cardiogenesis. Mutations disrupting trabecular development in the mouse are frequently embryonic lethal, testifying to the importance of the trabeculae, and aberrant trabecular structure is associated with several human cardiac pathologies. Here, trabecular architecture in the developing mouse embryo has been analysed using high-resolution episcopic microscopy (HREM) and three-dimensional (3D) modelling. This study shows that at all stages from mid-gestation to birth, the ventricular trabeculae comprise a complex meshwork of myocardial strands. Such an arrangement defies conventional methods of measurement, and an approach based upon fractal algorithms has been used to provide an objective measure of trabecular complexity. The extent of trabeculation as it changes along the length of left and right ventricles has been quantified, and the changes that occur from formation of the four-chambered heart until shortly before birth have been mapped. This approach not only measures qualitative features evident from visual inspection of 3D models, but also detects subtle, consistent and regionally localised differences that distinguish each ventricle and its developmental stage. Finally, the combination of HREM imaging and fractal analysis has been applied to analyse changes in embryonic heart structure in a genetic mouse model in which trabeculation is deranged. It is shown that myocardial deletion of the Notch pathway component Mib1 (Mib1(flox/flox); cTnT-cre) results in a complex array of abnormalities affecting trabeculae and other parts of the heart. J Anat. 2016; 229(2):314-25 10.1111/joa.12465 1469-7580 0021-8782 Journal of Anatomy 27020702 http://hdl.handle.net/20.500.12105/5206 Cardiac embryology Cardiogenesis Developmental biology Non-compaction cardiomyopathy VENTRICULAR NONCOMPACTION CARDIOMYOPATHY RESOLUTION EPISCOPIC MICROSCOPY CONDUCTION SYSTEM HEART DEVELOPMENT NON-COMPACTION PROLIFERATION ARCHITECTURE EXPRESSION MUTATIONS Morphogenesis of myocardial trabeculae in the mouse embryo