2026-06-14T03:32:26Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/52052024-09-27T10:06:55Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607
Repisalud
author
Bujak, Renata
author
Mateo, Jesus
author
Blanco, Isabel
author
Izquierdo-Garcia, Jose L.
author
Dudzik, Danuta
author
Markuszewski, Michal J.
author
Ivo Peinado, Victor
author
Laclaustra, Martin
author
Albert Barbera, Joan
author
Barbas, Coral
author
Ruiz-Cabello, Jesus
funder
National Science Centre (Polonia)
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Ministry of Science and Higher Education (Polonia)
funder
Fundación ProCNIC
2017-10-30T13:15:40Z
2017-10-30T13:15:40Z
2016
PLoS One. 2016; 11(8):e0160505
10.1371/journal.pone.0160505
1932-6203
Plos One
27486806
http://hdl.handle.net/20.500.12105/5205
Diagnosis of pulmonary arterial hypertension (PAH) is difficult due to the lack of specific clinical symptoms and biomarkers, especially at early stages. We compared plasma metabolic fingerprints of PAH patients (n = 20) with matched healthy volunteers (n = 20) using, for the first time, untargeted multiplatform metabolomics approach consisting of high-performance liquid and gas chromatography coupled with mass spectrometry. Multivariate statistical analyses were performed to select metabolites that contribute most to groups' classification (21 from liquid in both ionization modes and 9 from gas chromatography-mass spectrometry). We found metabolites related to energy imbalance, such as glycolysis-derived metabolites, as well as metabolites involved in fatty acid, lipid and amino acid metabolism. We observed statistically significant changes in threitol and aminomalonic acid in PAH patients, which could provide new biochemical insights into the pathogenesis of the disease. The results were externally validated on independent case and control cohorts, confirming up to 16 metabolites as statistically significant in the validation study. Multiplatform metabolomics, followed by multivariate chemometric data analysis has a huge potential for explaining pathogenesis of PAH and for searching potential and new more specific and less invasive markers of the disease.
eng
ALPHA PPAR-ALPHA
ENDOTHELIAL-CELLS
PATHWAY
GLUTAMINOLYSIS
METABOLISM
RELEVANCE
OXIDATION
PLASMA
ACID
New Biochemical Insights into the Mechanisms of Pulmonary Arterial Hypertension in Humans
journal article
URL
https://repisalud.isciii.es/bitstreams/c5d6aa60-ed18-40be-9f7f-09db5b9fd81d/download
File
MD5
72428fca4c3dbfb78942c49db77f9086
1618236
application/pdf
NewBiochemicalInsightsIntoTheMechanisms_2016
URL
https://repisalud.isciii.es/bitstreams/4aabb551-ef53-4567-90d1-d85ad804a1a1/download
File
MD5
79f335bf794aa170750d4741fa0cb572
44182
text/plain
NewBiochemicalInsightsIntoTheMechanisms_2016.txt