2026-06-14T03:44:10Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/51802024-09-27T09:15:31Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Hurtado-Roca, Yamilee
author
Ledesma, Marta
author
Gonzalez-Lazaro, Monica
author
Moreno-Loshuertos, Raquel
author
Fernandez-Silva, Patricio
author
Enriquez, Jose Antonio
author
Laclaustra, Martin
funder
Instituto de Salud Carlos III
funder
Ministerio de la Producción (Perú)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2017-10-20T10:33:52Z
2017-10-20T10:33:52Z
2016
PLoS One. 2016; 11(10):e0163770
10.1371/journal.pone.0163770
1932-6203
PLoS One
27736919
http://hdl.handle.net/20.500.12105/5180
Alterations of mitochondrial DNA copy number (mtDNAcn) in the blood (mitochondrial to nuclear DNA ratio) appear associated with several systemic diseases, including primary mitochondrial disorders, carcinogenesis, and hematologic diseases. Measuring mtDNAcn in DNA extracted from whole blood (WB) instead of from peripheral blood mononuclear cells or buffy coat may yield different results due to mitochondrial DNA present in platelets. The aim of this work is to quantify the contribution of platelets to mtDNAcn in whole blood [mtDNAcn(WB)] and to propose a correction formula to estimate leukocytes' mtDNAcn [mtDNAcn(L)] from mtDNAcn(WB). Blood samples from 10 healthy adults were combined with platelet-enriched plasma and saline solution to produce artificial blood preparations. Aliquots of each sample were combined with five different platelet concentrations. In 46 of these blood preparations, mtDNAcn was measured by qPCR. MtDNAcn(WB) increased 1.07 (95\% CI 0.86, 1.29; p<0.001) per 1000 platelets present in the preparation. We proved that leukocyte count should also be taken into account as mtDNAcn(WB) was inversely associated with leukocyte count; it increased 1.10 (95\% CI 0.95, 1.25, p<0.001) per unit increase of the ratio between platelet and leukocyte counts. If hematological measurements are available, subtracting 1.10 the platelets/leukocyte ratio from mtDNAcn(WB) may serve as an estimation for mtDNAcn(L). Both platelet and leukocyte counts in the sample are important sources of variation if comparing mtDNAcn among groups of patients when mtDNAcn is measured in DNA extracted from whole blood. Not taking the platelet/leukocyte ratio into account in whole blood measurements, may lead to overestimation and misclassification if interpreted as leukocytes' mtDNAcn.
eng
MITOCHONDRIAL-DNA CONTENT
HIV-INFECTED PATIENTS
COPY NUMBER
MONONUCLEAR-CELLS
LYMPHOMA
DISEASE
RISK
MUTATIONS
DEPLETION
DYNAMICS
Adjusting MtDNA Quantification in Whole Blood for Peripheral Blood Platelet and Leukocyte Counts
journal article
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https://repisalud.isciii.es/bitstreams/ea704d29-f431-4340-92bc-4081a8fd8750/download
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MD5
b60ba4182c0d68b675c8fdb106fda9c5
1161664
application/pdf
AdjustingMtDNAQuantificationIn_2016
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7f430e4c5ea03ca314999877a04964cc
45686
text/plain
AdjustingMtDNAQuantificationIn_2016.txt
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https://repisalud.isciii.es/bitstreams/1d8d8432-8fbf-47cb-852a-2d4509f2da4e/download
File
MD5
7f430e4c5ea03ca314999877a04964cc
45686
text/plain
AdjustingMtDNAQuantificationIn_2016.txt