2026-08-10T01:24:25Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/51702024-09-27T08:12:49Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
Burillo, Elena
author
Jorge, Inmaculada
author
Martinez-Lopez, Diego
author
Camafeita, Emilio
author
Miguel Blanco-Colio, Luis
author
Trevisan-Herraz, Marco
author
Ezkurdia, Iakes
author
Egido, Jesus
author
Michel, Jean-Baptiste
author
Meilhac, Olivier
author
Vazquez, Jesus
author
Luis Martin-Ventura, Jose
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (España)
funder
Fundación Renal Íñigo Álvarez de Toledo
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundación ProCNIC
2017-10-20T10:33:50Z
2017-10-20T10:33:50Z
2016
Sci Rep. 2016; 6:38477
10.1038/srep38477
2045-2322
Scientific Reports
27934969
http://hdl.handle.net/20.500.12105/5170
High-density lipoproteins (HDLs) are complex protein and lipid assemblies whose composition is known to change in diverse pathological situations. Analysis of the HDL proteome can thus provide insight into the main mechanisms underlying abdominal aortic aneurysm (AAA) and potentially detect novel systemic biomarkers. We performed a multiplexed quantitative proteomics analysis of HDLs isolated from plasma of AAA patients (N = 14) and control study participants (N = 7). Validation was performed by western-blot (HDL), immunohistochemistry (tissue), and ELISA (plasma). HDL from AAA patients showed elevated expression of peroxiredoxin-6 (PRDX6), HLA class I histocompatibility antigen (HLA-I), retinol-binding protein 4, and paraoxonase/arylesterase 1 (PON1), whereas alpha-2 macroglobulin and C4b-binding protein were decreased. The main pathways associated with HDL alterations in AAA were oxidative stress and immune-inflammatory responses. In AAA tissue, PRDX6 colocalized with neutrophils, vascular smooth muscle cells, and lipid oxidation. Moreover, plasma PRDX6 was higher in AAA (N = 47) than in controls (N = 27), reflecting increased systemic oxidative stress. Finally, a positive correlation was recorded between PRDX6 and AAA diameter. The analysis of the HDL proteome demonstrates that redox imbalance is a major mechanism in AAA, identifying the antioxidant PRDX6 as a novel systemic biomarker of AAA.
eng
HIGH-DENSITY-LIPOPROTEINS
NADPH OXIDASE ACTIVITY
COMPLEMENT ACTIVATION
OXIDATIVE STRESS
SMOOTH-MUSCLE
ATHEROSCLEROSIS
DISCOVERY
RUPTURE
CELLS
Quantitative HDL Proteomics Identifies Peroxiredoxin-6 as a Biomarker of Human Abdominal Aortic Aneurysm
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QuantitativeHDLProteomicsIdentifies_2016
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QuantitativeHDLProteomicsIdentifies_2016.txt
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QuantitativeHDLProteomicsIdentifies_2016.txt