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oai:repisalud.isciii.es:20.500.12105/51702024-09-27T08:12:49Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607

00925njm 22002777a 4500 dc Burillo, Elena author Jorge, Inmaculada author Martinez-Lopez, Diego author Camafeita, Emilio author Miguel Blanco-Colio, Luis author Trevisan-Herraz, Marco author Ezkurdia, Iakes author Egido, Jesus author Michel, Jean-Baptiste author Meilhac, Olivier author Vazquez, Jesus author Luis Martin-Ventura, Jose author 2016 High-density lipoproteins (HDLs) are complex protein and lipid assemblies whose composition is known to change in diverse pathological situations. Analysis of the HDL proteome can thus provide insight into the main mechanisms underlying abdominal aortic aneurysm (AAA) and potentially detect novel systemic biomarkers. We performed a multiplexed quantitative proteomics analysis of HDLs isolated from plasma of AAA patients (N = 14) and control study participants (N = 7). Validation was performed by western-blot (HDL), immunohistochemistry (tissue), and ELISA (plasma). HDL from AAA patients showed elevated expression of peroxiredoxin-6 (PRDX6), HLA class I histocompatibility antigen (HLA-I), retinol-binding protein 4, and paraoxonase/arylesterase 1 (PON1), whereas alpha-2 macroglobulin and C4b-binding protein were decreased. The main pathways associated with HDL alterations in AAA were oxidative stress and immune-inflammatory responses. In AAA tissue, PRDX6 colocalized with neutrophils, vascular smooth muscle cells, and lipid oxidation. Moreover, plasma PRDX6 was higher in AAA (N = 47) than in controls (N = 27), reflecting increased systemic oxidative stress. Finally, a positive correlation was recorded between PRDX6 and AAA diameter. The analysis of the HDL proteome demonstrates that redox imbalance is a major mechanism in AAA, identifying the antioxidant PRDX6 as a novel systemic biomarker of AAA. Sci Rep. 2016; 6:38477 10.1038/srep38477 2045-2322 Scientific Reports 27934969 http://hdl.handle.net/20.500.12105/5170 HIGH-DENSITY-LIPOPROTEINS NADPH OXIDASE ACTIVITY COMPLEMENT ACTIVATION OXIDATIVE STRESS SMOOTH-MUSCLE ATHEROSCLEROSIS DISCOVERY RUPTURE CELLS Quantitative HDL Proteomics Identifies Peroxiredoxin-6 as a Biomarker of Human Abdominal Aortic Aneurysm