2026-08-05T15:42:57Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/51582024-09-27T08:52:42Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Villa-Bellosta, Ricardo
author
Hamczyk, Magda R.
author
Andres, Vicente
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundación ProCNIC
2017-10-20T10:33:49Z
2017-10-20T10:33:49Z
2017
PLoS One. 2017; 12(3):e0174998
10.1371/journal.pone.0174998
1932-6203
PLoS One
28362852
http://hdl.handle.net/20.500.12105/5158
Purpose Phosphorus is an essential nutrient involved in many pathobiological processes. Less than 1\% of phosphorus is found in extracellular fluids as inorganic phosphate ion (Pi) in solution. High serum Pi level promotes ectopic calcification in many tissues, including blood vessels. Here, we studied the effect of elevated Pi concentration on macrophage polarization and calcification. Macrophages, present in virtually all tissues, play key roles in health and disease and display remarkable plasticity, being able to change their physiology in response to environmental cues. Methods and results High-throughput transcriptomic analysis and functional studies demonstrated that Pi induces unpolarized macrophages to adopt a phenotype closely resembling that of alternatively -activated M2 macrophages, as revealed by arginine hydrolysis and energetic and antioxidant profiles. Pi-induced macrophages showed an anti-calcifying action mediated by increased availability of extracellular ATP and pyrophosphate. Conclusion We conclude that the ability of Pi-activated macrophages to prevent calcium-phosphate deposition is a compensatory mechanism protecting tissues from hyperphosphatemiainduced pathologic calcification.
eng
VASCULAR SMOOTH-MUSCLE
CHRONIC KIDNEY-DISEASE
IN-VITRO
MEDIATED INFLAMMATION
CELL CALCIFICATION
METABOLISM
POLARIZATION
CALCIUM
ATHEROSCLEROSIS
HOMEOSTASIS
Novel phosphate-activated macrophages prevent ectopic calcification by increasing extracellular ATP and pyrophosphate
journal article
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55e634fc612a8c12cf6a67028e5d459a
1893043
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NovelPhosphate-ActivatedMacrophages _2017
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45037
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NovelPhosphate-ActivatedMacrophages _2017.txt