2026-08-05T15:43:18Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/51582024-09-27T08:52:42Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Villa-Bellosta, Ricardo author Hamczyk, Magda R. author Andres, Vicente author 2017 Purpose Phosphorus is an essential nutrient involved in many pathobiological processes. Less than 1\% of phosphorus is found in extracellular fluids as inorganic phosphate ion (Pi) in solution. High serum Pi level promotes ectopic calcification in many tissues, including blood vessels. Here, we studied the effect of elevated Pi concentration on macrophage polarization and calcification. Macrophages, present in virtually all tissues, play key roles in health and disease and display remarkable plasticity, being able to change their physiology in response to environmental cues. Methods and results High-throughput transcriptomic analysis and functional studies demonstrated that Pi induces unpolarized macrophages to adopt a phenotype closely resembling that of alternatively -activated M2 macrophages, as revealed by arginine hydrolysis and energetic and antioxidant profiles. Pi-induced macrophages showed an anti-calcifying action mediated by increased availability of extracellular ATP and pyrophosphate. Conclusion We conclude that the ability of Pi-activated macrophages to prevent calcium-phosphate deposition is a compensatory mechanism protecting tissues from hyperphosphatemiainduced pathologic calcification. PLoS One. 2017; 12(3):e0174998 10.1371/journal.pone.0174998 1932-6203 PLoS One 28362852 http://hdl.handle.net/20.500.12105/5158 VASCULAR SMOOTH-MUSCLE CHRONIC KIDNEY-DISEASE IN-VITRO MEDIATED INFLAMMATION CELL CALCIFICATION METABOLISM POLARIZATION CALCIUM ATHEROSCLEROSIS HOMEOSTASIS Novel phosphate-activated macrophages prevent ectopic calcification by increasing extracellular ATP and pyrophosphate