2026-06-14T12:12:53Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/51112024-09-27T09:55:04Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
Gutierrez-Vazquez, Cristina
author
Rodriguez-Galan, Ana
author
Fernandez-Alfara, Marcos
author
Mittelbrunn, Maria
author
Sanchez-Cabo, Fatima
author
Jorge Martinez-Herrera, Dannys
author
Ramirez-Huesca, Marta
author
Pascual-Montano, Alberto
author
Sanchez-Madrid, Francisco
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Fundación ProCNIC
2017-10-20T10:23:11Z
2017-10-20T10:23:11Z
2017
Sci Rep. 2017; 7(1):3508
https://doi.org/10.1038/s41598-017-03689-7
2045-2322
Scientific Report
28615644
http://hdl.handle.net/20.500.12105/5111
microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3'UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells.
eng
MESSENGER-RNA
MICRORNA
EXPRESSION
DIFFERENTIATION
PI3K
PREDICTION
P85-ALPHA
DUPLEXES
DICER
miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p
journal article
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miRNAProfilingDuringAntigen-dependent_2017
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miRNAProfilingDuringAntigen-dependent_2017_Supp
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miRNAProfilingDuringAntigen-dependent_2017.txt
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miRNAProfilingDuringAntigen-dependent_2017_Supp.txt
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miRNAProfilingDuringAntigen-dependent_2017.txt
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miRNAProfilingDuringAntigen-dependent_2017_Supp.txt