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oai:repisalud.isciii.es:20.500.12105/51112024-09-27T09:55:04Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607

00925njm 22002777a 4500 dc Gutierrez-Vazquez, Cristina author Rodriguez-Galan, Ana author Fernandez-Alfara, Marcos author Mittelbrunn, Maria author Sanchez-Cabo, Fatima author Jorge Martinez-Herrera, Dannys author Ramirez-Huesca, Marta author Pascual-Montano, Alberto author Sanchez-Madrid, Francisco author 2017 microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3'UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells. Sci Rep. 2017; 7(1):3508 https://doi.org/10.1038/s41598-017-03689-7 2045-2322 Scientific Report 28615644 http://hdl.handle.net/20.500.12105/5111 MESSENGER-RNA MICRORNA EXPRESSION DIFFERENTIATION PI3K PREDICTION P85-ALPHA DUPLEXES DICER miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p