2026-06-14T03:40:39Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/51072024-10-31T11:52:52Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
Gonzalez-Calero, Laura
author
Martinez, Paula J.
author
Martin-Lorenzo, Marta
author
Baldan-Martin, Montserrat
author
Ruiz-Hurtado, Gema
author
de la Cuesta, Fernando
author
Calvo, Eva
author
Segura, Julian
author
Lopez, Juan Antonio
author
Vazquez, Jesus
author
Barderas, Maria G
author
Ruilope, Luis M
author
Vivanco, Fernando
author
Alvarez-Llamas, Gloria
funder
Instituto de Salud Carlos III
funder
Fundación SENEFRO
funder
Fundación Conchita Rábago de Jiménez Díaz
funder
Redes Temáticas de Investigación Cooperativa en Salud (RETICS) (España)
2017-10-20T10:23:10Z
2017-10-20T10:23:10Z
2017
Oncotarget. 2017; 8(27):44217-44231
10.18632/oncotarget.17787
1949-2553
Oncotarget
28562335
http://hdl.handle.net/20.500.12105/5107
Albuminuria is an indicator of cardiovascular risk and renal damage in hypertensive individuals. Chronic renin-angiotensin system (RAS) suppression facilitates blood pressure control and prevents development of new-onset-albuminuria. A significant number of patients, however, develop albuminuria despite chronic RAS blockade, and the physiopathological mechanisms are underexplored. Urinary exosomes reflect pathological changes taking place in the kidney. The objective of this work was to examine exosomal protein alterations in hypertensive patients with albuminuria in the presence of chronic RAS suppression, to find novel clues underlying its development. Patients were followed-up for three years and were classified as: a) patients with persistent normoalbuminuria; b) patients developing de novo albuminuria; and c) patients with maintained albuminuria. Exosomal protein alterations between groups were identified by isobaric tag quantitation (iTRAQ). Confirmation was approached by target analysis (SRM). In total, 487 proteins were identified with high confidence. Specifically, 48 proteins showed an altered pattern in response to hypertension and/or albuminuria. Out of them, 21 proteins interact together in three main functional clusters: glycosaminoglycan degradation, coagulation and complement system, and oxidative stress. The identified proteins constitute potential targets for drug development and may help to define therapeutic strategies to evade albuminuria progression in hypertensive patients chronically treated.
eng
Exosomes
Hypertension
Albuminuria
Renin-angiotensin system
Proteomics
CHRONIC KIDNEY-DISEASE
DIABETIC-NEPHROPATHY
HEPARAN-SULFATE
COMPLEMENT C3
EXTRACELLULAR VESICLES
PEPTIDE IDENTIFICATION
SYSTEM SUPPRESSION
PROTEOMIC ANALYSIS
EPITHELIAL-CELLS
PROGNOSTIC VALUE
Urinary exosomes reveal protein signatures in hypertensive patients with albuminuria
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UrinaryExosomesRevealProtein_2017
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UrinaryExosomesRevealProtein_2017_Table1and2
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UrinaryExosomesRevealProtein_2017.txt
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UrinaryExosomesRevealProtein_2017.txt
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UrinaryExosomesRevealProtein_2017_Supp.txt
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UrinaryExosomesRevealProtein_2017_Table1and2.txt