2026-04-29T04:09:36Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/51062024-10-31T11:41:16Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Perez-Garcia, Arantxa
author
Marina-Zarate, Ester
author
Alvarez-Prado, Angel Francisco
author
Ligos, Jose M.
author
Galjart, Niels
author
Ramiro, Almudena R
funder
Ministerio de Educación, Cultura y Deporte (España)
funder
Ministerio de Economía y Competitividad (España)
funder
Centro Nacional de Investigaciones Cardiovasculares Carlos III (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Fundación ProCNIC
funder
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
2017-10-20T10:23:10Z
2017-10-20T10:23:10Z
2017
Nat Commun. 2017; 8:16067
2041-1723
http://hdl.handle.net/20.500.12105/5106
28677680
10.1038/ncomms16067
Nature Communications
In germinal centres (GC) mature B cells undergo intense proliferation and immunoglobulin gene modification before they differentiate into memory B cells or long-lived plasma cells (PC). GC B-cell-to-PC transition involves a major transcriptional switch that promotes a halt in cell proliferation and the production of secreted immunoglobulins. Here we show that the CCCTC-binding factor (CTCF) is required for the GC reaction in vivo, whereas in vitro the requirement for CTCF is not universal and instead depends on the pathways used for B-cell activation. CTCF maintains the GC transcriptional programme, allows a high proliferation rate, and represses the expression of Blimp-1, the master regulator of PC differentiation. Restoration of Blimp-1 levels partially rescues the proliferation defect of CTCF-deficient B cells. Thus, our data reveal an essential function of CTCF in maintaining the GC transcriptional programme and preventing premature PC differentiation.
eng
3' REGULATORY REGION
C-MYC GENE
IGH LOCUS
T-CELLS
B-CELLS
CHROMATIN ARCHITECTURE
V(D)J RECOMBINATION
GENOME
EXPRESSION
BLIMP-1
CTCF orchestrates the germinal centre transcriptional program and prevents premature plasma cell differentiation
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