2026-08-29T08:18:12Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/51002024-09-27T09:53:54Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
Pontes-Quero, Samuel
author
Heredia, Luis
author
Casquero-Garcia, Veronica
author
Fernandez-Chacon, Macarena
author
Luo, Wen
author
Hermoso, Ana
author
Bansal, Mayank
author
Garcia-Gonzalez, Irene
author
Sanchez-Munoz, Maria S.
author
Perea, Juan R.
author
Galiana-Simal, Adrian
author
Rodriguez-Arabaolaza, Iker
author
Del Olmo-Cabrera, Sergio
author
Rocha, Susana
author
Criado-Rodriguez, Luis M.
author
Giovinazzo, Giovanna
author
Benedito, Rui
funder
Ministerio de Economía y Competitividad (España)
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Fundación La Caixa
funder
Fundación ProCNIC
2017-10-20T10:23:09Z
2017-10-20T10:23:09Z
2017
Cell. 2017; 170(4):800-814 e18
10.1016/j.cell.2017.07.031
1097-4172
0092-8674
Cell
28802047
http://hdl.handle.net/20.500.12105/5100
Improved methods for manipulating and analyzing gene function have provided a better understanding of how genes work during organ development and disease. Inducible functional genetic mosaics can be extraordinarily useful in the study of biological systems; however, this experimental approach is still rarely used in vertebrates. This is mainly due to technical difficulties in the assembly of large DNA constructs carrying multiple genes and regulatory elements and their targeting to the genome. In addition, mosaic phenotypic analysis, unlike classical single gene-function analysis, requires clear labeling and detection of multiple cell clones in the same tissue. Here, we describe several methods for the rapid generation of transgenic or gene-targeted mice and embryonic stem (ES) cell lines containing all the necessary elements for inducible, fluorescent, and functional genetic mosaic (ifgMosaic) analysis. This technology enables the interrogation of multiple and combinatorial gene function with high temporal and cellular resolution.
eng
CRE-LOXP
IN-VIVO
MOUSE
MICE
EXPRESSION
RECOMBINATION
SYSTEM
Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis
journal article
URL
https://repisalud.isciii.es/bitstreams/ee911527-3d58-49ca-a6bd-34b39efd9cb0/download
File
MD5
868138a1c668100f70912ea4007f0d57
14054080
application/pdf
ResourceDualifgMosaic:AVersatileMethod_2017
URL
https://repisalud.isciii.es/bitstreams/95446813-e7c5-43a9-abb5-f94e18f8fd53/download
File
MD5
de83d249dd1a531a2a4e7f93f2e6d79c
112729
text/plain
ResourceDualifgMosaic:AVersatileMethod_2017.txt