2026-06-14T00:27:35Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/48602025-02-27T17:40:27Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Martin-Martin, Ines
author
Jimenez, Maribel
author
Gonzalez, Estela
author
Eguiluz, Cesar
author
Molina, Ricardo
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Comisión Europea. 7 Programa Marco
2017-09-04T16:36:04Z
2017-09-04T16:36:04Z
2015-12-09
Vet Res. 2015; 46:138
10.1186/s13567-015-0281-1
1297-9716
Veterinary Research
http://hdl.handle.net/20.500.12105/4860
A human leishmaniasis outbreak is occurring in the Madrid region, Spain, with the parasite and vector involved being Leishmania infantum and Phlebotomus perniciosus respectively. The aim of this study was to investigate the virulence of L. infantum isolates from the focus using a natural transmission model. Hamsters were infected by intraperitoneal inoculation (IP) or by bites of sand flies experimentally infected with L. infantum isolates obtained from P. perniciosus collected in the outbreak area (IPER/ES/2012/BOS1FL1 and IPER/ES/2012/POL2FL6) and a well characterized L. infantum strain JPCM5 (MCAN/ES/98/LLM-877). Hamster infections were monitored by clinical examination, serology, culture, parasite burden, Giemsa-stained imprints, PCR, histopathology and xenodiagnostic studies. Establishment of infection of L. infantum was achieved with the JPCM5 strain and outbreak isolates by both P. perniciosus infective bites or IP route. However, high virulence of BOS1FL1 and POL2FL6 isolates was highlighted by the clinical outcome of disease, high parasite detection in spleen and liver, high parasitic loads and positivity of Leishmania serology. Transmission by bite of POL2FL6 infected flies generated a slower progression of clinical disease than IP infection, but both groups were infective to P. perniciosus by xenodiagnosis at 2 months post-infection. Conversely, hamsters inoculated with JPCM5 were not infective to sand flies. Histopathology studies confirmed the wide spread of POL2FL6 parasites to several organs. A visceral leishmaniasis model that mimics the natural transmission in nature allowed us to highlight the high virulence of isolates that are circulating in the focus. These findings contribute to a better understanding of the outbreak epidemiology.
eng
Animals
female
Humans
Leishmania infantum
Natural transmission of Leishmania infantum through experimentally infected Phlebotomus perniciosus highlights the virulence of Leishmania parasites circulating in the human visceral leishmaniasis outbreak in Madrid, Spain
research article
URL
https://repisalud.isciii.es/bitstreams/4c5dfad3-4aa1-49f9-9bb9-73f159e20339/download
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NaturalTransmissionOfLeishmania_2015
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https://repisalud.isciii.es/bitstreams/97efa416-7db3-4ca1-9a9b-abc976f23dd7/download
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text/plain
13567_2015_Article_281.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/5893b956-45b8-4a39-a414-55d70144ed56/download
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NaturalTransmissionOfLeishmania_2015.txt
URL
https://repisalud.isciii.es/bitstreams/6c21a5b0-c57f-4fff-84a7-b77b663a3871/download
File
MD5
56e59f4d07d936b6656dfd77b3d4d260
62067
text/plain
NaturalTransmissionOfLeishmania_2015.txt