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oai:repisalud.isciii.es:20.500.12105/48552025-06-23T18:35:46Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud author Remoli, Maria Elena author Jimenez, Maribel author Fortuna, Claudia author Benedetti, Eleonora author Marchi, Antonella author Genovese, Domenico author Gramiccia, Marina author Molina, Ricardo author Ciufolini, Maria Grazia funder Unión Europea. Comisión Europea. 7 Programa Marco 2017-09-04T16:35:16Z 2017-09-04T16:35:16Z 2016-04-13 Parasit Vectors. 2016; 9: 205 10.1186/s13071-016-1488-3 1756-3305 Parasites & Vectors 27075742 http://hdl.handle.net/20.500.12105/4855 Background: Phlebotomus-borne (PhB-) viruses are distributed in large areas of the Old World and are widespread throughout the Mediterranean basin, where recent investigations have indicated that virus diversity is higher than initially suspected. Some of these viruses are causes of meningitis, encephalitis and febrile illnesses. In order to monitor the viral presence and the infection rate of PhB-viruses in a recently identified and well characterized human zoonotic leishmaniasis focus in southwestern Madrid, Spain, a sand fly collection was carried out. Methods: Sand fly insects were collected in four stations using CDC light traps during 2012-2013 summer seasons. Screening for Phlebovirus presence both via isolation on Vero cells and via polymerase chain reaction (PCR), using degenerated primers targeting a portion of the L segment, was performed. The serological identity and phylogenetic relationships on the three genomic segments of the viral isolates were carried out. Results: Six viral isolates belonging to different serological complexes of the genus Phlebovirus were obtained from fifty pools on a total of 963 P. perniciosus (202 females). Phylogenetic analysis and serological assays allowed the identification of two isolates of Toscana virus (TOSV) B genotype, three isolates strongly related to Italian Arbia virus (ARBV), and one isolate of a novel putative Phlebovirus related to the recently characterized Arrabida virus in South Portugal, tentatively named Arrabida-like virus. Positive male sand fly pools suggested that transovarial or venereal transmission could occur under natural conditions. Conclusions: Our findings highlighted the presence of different Phlebovirus species in the South-West area of the Madrid Autonomous Community where an outbreak of cutaneous and visceral human leishmaniasis has been recently described. The evidence of viral species never identified before in Spain, as ARBV and Arrabida-like virus, and TOSV B genotype focus stability was demonstrated. Environmental aspects such as climate change, growing urbanization, socio-economic development could have contributed to the genesis of this wide ecological niche of PhB-viruses and Leishmania spp. The potential role of vertebrates as reservoir for the phleboviruses identified and the possibility of Phleboviruses-Leishmania co-infection in the same sand fly should be assessed. Furthermore the PhB-viruses impact on human health should be implemented. eng Phlebovirus Phlebotomus perniciosus Toscana virus Human leishmaniasis Arbia virus Novel virus Madrid Spain Phleboviruses detection in Phlebotomus perniciosus from a human leishmaniasis focus in South-West Madrid region, Spain research article URL https://repisalud.isciii.es/bitstreams/f17ed306-f3b0-43df-9a43-b84a320b9285/download File MD5 5287ea7270ee18ec9a4d4c3a939bd05e 1408642 application/pdf PhlebovirusesDetectionInPhlebotomus_2016 URL https://repisalud.isciii.es/bitstreams/8b016ef5-a57f-4081-ad0a-2c99ae2e3832/download File MD5 f78a080f00106d65cc696952b40aed6d 53099 text/plain 13071_2016_Article_1488.pdf.txt URL https://repisalud.isciii.es/bitstreams/929569b1-e1df-4aae-968a-1cde477803e2/download File MD5 f78a080f00106d65cc696952b40aed6d 53099 text/plain PhlebovirusesDetectionInPhlebotomus_2016.txt URL https://repisalud.isciii.es/bitstreams/29d23afe-efc6-4511-b0b8-14a123d1e6b6/download File MD5 f78a080f00106d65cc696952b40aed6d 53099 text/plain PhlebovirusesDetectionInPhlebotomus_2016.txt