2026-06-14T10:37:36Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/48432025-04-08T09:27:31Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2052col_20.500.12105_16970col_20.500.12105_19616
Repisalud
author
Toraño, Estela G
author
Bayón, Gustavo F
author
del Real, Álvaro
author
Sierra, Marta I
author
García, María G
author
Carella, Antonella
author
Belmonte, Thalia
author
Urdinguio, Rocio G
author
Cubillo, Isabel
author
Garcia-Castro, Javier
author
Delgado-Calle, Jesús
author
Pérez-Campo, Flor M
author
Riancho, José A
author
Fraga, Mario F
author
Fernandez, Agustín F
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Instituto de Salud Carlos III
funder
Asociación Española Contra el Cáncer
funder
Fundación Ramón Areces
funder
Comunidad de Madrid (España)
funder
Gobierno del Principado de Asturias (España)
funder
Fundación Cajastur
2017-09-04T16:33:19Z
2017-09-04T16:33:19Z
2016-07-08
J Transl Med. 2016; 14: 207
10.1186/s12967-016-0966-x
1479-5876
Journal of Translational Medicine
http://hdl.handle.net/20.500.12105/4843
BACKGROUND: Age-associated changes in genomic DNA methylation have been primarily attributed to 5-methylcytosine (5mC). However, the recent discovery of 5-hydroxymethylcytosine (5hmC) suggests that this epigenetic mark might also play a role in the process. METHODS: Here, we analyzed the genome-wide profile of 5hmc in mesenchymal stem cells (MSCs) obtained from bone-marrow donors, aged 2-89 years. RESULTS: We identified 10,685 frequently hydroxymethylated CpG sites in MSCs that were, as in other cell types, significantly associated with low density CpG regions, introns, the histone posttranslational modification H3k4me1 and enhancers. Study of the age-associated changes to 5hmC identified 785 hyper- and 846 hypo-hydroxymethylated CpG sites in the MSCs obtained from older individuals.
CONCLUSIONS: DNA hyper-hydroxymethylation in the advanced-age group was associated with loss of 5mC, which suggests that, at specific CpG sites, this epigenetic modification might play a role in DNA methylation changes during lifetime. Since bone-marrow MSCs have many clinical applications, and the fact that the epigenomic alterations in this cell type associated with aging identified in this study could have associated functional effects, the age of donors should be taken into account in clinical settings.
eng
5hmC
MSCs
Aging
Epigenetics
Bone-marrow
Age-associated hydroxymethylation in human bone-marrow mesenchymal stem cells
research article
URL
https://repisalud.isciii.es/bitstreams/6a35fa7d-94e7-4719-abcb-2f484c2a4ca5/download
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AgeAssociatedHydroxymethylationIn_2016
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https://repisalud.isciii.es/bitstreams/74770630-9144-4dec-8a9f-5640382b9f08/download
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text/plain
12967_2016_Article_966.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/13b73f13-d4ab-41d8-ac8d-2276d720db0d/download
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AgeAssociatedHydroxymethylationIn_2016.txt
URL
https://repisalud.isciii.es/bitstreams/4417759d-3cf6-4108-a371-866dc5fbf121/download
File
MD5
a64d22bffbe841af19e79c326beca5d7
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AgeAssociatedHydroxymethylationIn_2016.txt