2026-06-14T03:29:06Zhttps://repisalud.isciii.es/rest/oai/request
oai:repisalud.isciii.es:20.500.12105/48282025-05-05T18:50:43Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19611
Repisalud author Berzosa, Pedro author Mula Lozano, Patricia author Ramos Rincón, Jose Manuel author Garcia, Luz author Reyes, Francisco author Benito, Agustin funder RETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España) funder Instituto de Salud Carlos III 2017-09-04T16:30:57Z 2017-09-04T16:30:57Z 2015-09-18 Malar J. 2015;14:357 10.1186/s12936-015-0893-y 1475-2875 Malaria Journal 26383920 http://hdl.handle.net/20.500.12105/4828 BACKGROUND: Approximately 50 million people (60 %) live in malaria risk areas in Ethiopia, at altitudes below 2000 m. According to official data, 60-70 % of malaria cases are due to Plasmodium falciparum, and 40-30 % by Plasmodium vivax. The species Plasmodium ovale was detected in 2013 in the northwest of the country, being the first report of the presence of this species in Ethiopia since the 60 s. The aim of this study was to assess the diagnosis by microscopy and PCR, and demonstrate the presence of other Plasmodium species in the country. METHODS: The survey was conducted in Bulbula, situated in the Rift Valley (West Arsi Province, Oromia Region). From December 2010 to October 2011, 3060 samples were collected from patients with symptoms of malaria; the diagnosis of malaria was done by microscopy and confirmation by PCR. RESULTS: 736 samples were positive for malaria by microscopy. After removing the 260 samples (109 positives and 151 negatives) for which it was not possible to do PCR, there were a total of 2800 samples, 1209 are used for its confirmation by PCR and quality control (627 are positives and 582 negatives by microscopy). From the 627 positive samples, 604 were confirmed as positive by PCR, 23 false positives were detected, and the group of 582 negative samples, 184 were positive by PCR (false negatives), which added to the previous positive samples is a total of 788, positive samples for some species of Plasmodium sp. 13.3 % more positives were detected with the PCR than the microscopy. Importantly, 23 samples were detected by PCR as P. ovale, after the sequencing of these samples was determined as P. ovale curtisi. CONCLUSIONS: The PCR detected more positive samples than the microscopy; in addition, P. ovale and P. ovale/P. vivax were detected that had not been detected by microscopy, which can affect in the infection control. eng Malaria Plasmodium Microscopy diagnoses P. ovale PCR diagnoses Sequencing Quality of malaria diagnosis and molecular confirmation of Plasmodium ovale curtisi in a rural area of the southeastern region of Ethiopia research article URL https://repisalud.isciii.es/bitstreams/7f50a720-ebd2-4d36-968d-8462bc1650c2/download File MD5 fab0b16071120955692e127627603c29 1379268 application/pdf QualityOfMalariaDiagnosis_2015.pdf URL https://repisalud.isciii.es/bitstreams/ec19de30-f9c8-4568-af60-d31978ae556e/download File MD5 0861486006496c590dcd4dd15da880cf 32468 text/plain 12936_2015_Article_893.pdf.txt URL https://repisalud.isciii.es/bitstreams/a3d7a3da-f694-4658-ab9e-255386cb338a/download File MD5 0861486006496c590dcd4dd15da880cf 32468 text/plain QualityOfMalariaDiagnosis_2015.pdf.txt URL https://repisalud.isciii.es/bitstreams/b1cd5202-c346-4cff-8698-85ed9cf406c2/download File MD5 0861486006496c590dcd4dd15da880cf 32468 text/plain QualityOfMalariaDiagnosis_2015.pdf.txt