2026-07-21T15:38:59Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/48052025-06-23T18:36:47Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Villalón, Pilar
author
Valdezate, Sylvia
author
Cabezas, Teresa
author
Ortega, Montserrat
author
Garrido, Noelia
author
Vindel, Ana
author
Medina-Pascual, Maria Jose
author
Saez-Nieto, Juan Antonio
2017-09-04T16:27:15Z
2017-09-04T16:27:15Z
2015-02-25
BMC Microbiol. 2015; 15: 47
10.1186/s12866-015-0383-y
1471-2180
BMC Microbiology
25887224
http://hdl.handle.net/20.500.12105/4805
Background: Nosocomial outbreaks of multidrug-resistant Acinetobacter baumannii are of worldwide concern. Using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and multiple locus variable number tandem repeat sequence (VNTR) analysis (MLVA), the present work examines the genetic diversity of the endemic and epidemic A. baumannii clones isolated in a single hospital over a twelve-year period. Results: PFGE analysis of 405 A. baumannii-calcoaceticus complex isolates detected 15 A. baumannii endemic/epidemic PFGE types (EE1 to EE15) that grouped into five clusters: EE1-EE8, EE9, EE10, EE11 and EE12-EE15. The MLST sequence type (ST) distributions were: international clone II (ST-2) 60%, international clone III (ST-3) 26.7%, ST-15 6.7%, and ST-80 6.7%. MLVA-8Orsay returned 17 allelic profiles. The large (L) VNTR marker profiles were fully concordant with the detected STs, and concordant with 14 up to 15 PFGE types. Imipenem resistance was detected in five PFGE types; the prevalence of the bla OXA-58-like and bla OXA-40-like genes was 60% and 40% respectively. Conclusions: PFGE proved to be a vital tool for analysis of the temporal and spatial distribution of the clones. MLST and the VNTR L-markers grouped the isolates into clonal clusters. The wide diversity of MLVA small (S)-markers, however, did not permit clustering. The present results demonstrate the persistence of several endemic PFGE types in the hospital, the involvement of some of them in outbreaks, and the inter hospital transmission of extensively drug-resistant ST-15 and ST-80.
eng
A. baumannii
PFGE
Sequence type
MLVA
Clone
Endemic and epidemic Acinetobacter baumannii clones: a twelve-year study in a tertiary care hospital
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EndemicAndEpidemicAcinetobacter_2015.pdf
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12866_2015_Article_383.pdf.txt
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EndemicAndEpidemicAcinetobacter_2015.pdf.txt
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EndemicAndEpidemicAcinetobacter_2015.pdf.txt