2026-08-29T15:33:58Zhttps://repisalud.isciii.es/rest/oai/request
oai:repisalud.isciii.es:20.500.12105/47952025-05-05T09:35:21Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19611
Repisalud author Cordon-Obras, Carlos author Ochando, Jordi author Knapp, Jenny author Nebreda, Paloma author Ndong-Mabale, Nicolas author Ncogo, Policarpo author Ndongo-Asumu, Pedro author Navarro, Miguel author Pinto, João author Benito, Agustin author Bart, Jean Mathieu funder Instituto de Salud Carlos III 2017-09-04T16:25:38Z 2017-09-04T16:25:38Z 2014-01-17 Parasit Vectors. 2014; 7: 31 10.1186/1756-3305-7-31 1756-3305 Parasites & Vectors 24438585 http://hdl.handle.net/20.500.12105/4795 BACKGROUND: Luba is one of the four historical foci of Human African Trypanosomiasis (HAT) on Bioko Island, in Equatorial Guinea. Although no human cases have been detected since 1995, T. b. gambiense was recently observed in the vector Glossina palpalis palpalis. The existence of cryptic species within this vector taxon has been previously suggested, although no data are available regarding the evolutionary history of tsetse flies populations in Bioko. METHODS: A phylogenetic analysis of 60 G. p. palpalis from Luba was performed sequencing three mitochondrial (COI, ND2 and 16S) and one nuclear (rDNA-ITS1) DNA markers. Phylogeny reconstruction was performed by Distance Based, Maximum Likelihood and Bayesian Inference methods. RESULTS: The COI and ND2 mitochondrial genes were concatenated and revealed 10 closely related haplotypes with a dominant one found in 61.1% of the flies. The sequence homology of the other 9 haplotypes compared to the former ranged from 99.6 to 99.9%. Phylogenetic analysis clearly clustered all island samples with flies coming from the Western African Clade (WAC), and separated from the flies belonging to the Central Africa Clade (CAC), including samples from Mbini and Kogo, two foci of mainland Equatorial Guinea. Consistent with mitochondrial data, analysis of the microsatellite motif present in the ITS1 sequence exhibited two closely related genotypes, clearly divergent from the genotypes previously identified in Mbini and Kogo. CONCLUSIONS: We report herein that tsetse flies populations circulating in Equatorial Guinea are composed of two allopatric subspecies, one insular and the other continental. The presence of these two G. p. palpalis cryptic taxa in Equatorial Guinea should be taken into account to accurately manage vector control strategy, in a country where trypanosomiasis transmission is controlled but not definitively eliminated yet. eng Glossina palpalis palpalis Luba Equatorial Guinea Tsetse Trypanosomiasis Phylogeny Bioko Glossina palpalis palpalis populations from Equatorial Guinea belong to distinct allopatric clades research article URL https://repisalud.isciii.es/bitstreams/9d3539c3-a1e8-4212-8abd-d587a7a0b900/download File MD5 0644948e23e65fdc85fd3107c8eea45b 1252319 application/pdf GlossinaPalpalisPalpalisPopulations_2014.pdf URL https://repisalud.isciii.es/bitstreams/763d6e98-23f0-4cf0-99ae-bc62c3168842/download File MD5 85c60e9a63540f9483cd6e3e1b7ab3e9 49644 text/plain 13071_2013_Article_1179.pdf.txt URL https://repisalud.isciii.es/bitstreams/e74c8ada-8c39-4c9d-991f-131b8cf8e5d3/download File MD5 85c60e9a63540f9483cd6e3e1b7ab3e9 49644 text/plain GlossinaPalpalisPalpalisPopulations_2014.pdf.txt URL https://repisalud.isciii.es/bitstreams/0bf613ad-df11-4215-8e9e-86eb2ef6b2e3/download File MD5 6b95925919bf1ce677a35136f550b6c7 49629 text/plain GlossinaPalpalisPalpalisPopulations_2014.pdf.txt