2026-04-29T07:22:53Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/47942025-03-06T09:26:06Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Carrillo, Eugenia
author
Sanchez Herrero, Carmen
author
Cruz, Israel
author
Moreno, Javier
author
Cunha, Joana
author
Cordeiro-da-Silva, Anabela
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía y Competitividad (España)
2017-09-04T16:25:27Z
2017-09-04T16:25:27Z
2013-04-26
Parasit Vectors. 2013; 6: 122
http://hdl.handle.net/20.500.12105/4794
23622683
10.1186/1756-3305-6-122
1756-3305
Parasites & Vectors
BACKGROUND: Leishmaniasis is a group of diseases with a variety of clinical manifestations. The form of the disease is highly dependent on the infective Leishmania species and the immunological status of the host. The infectivity of the parasite strain also plays an important role in the progression of the infection. The aim of this work is to understand the influence of the natural infectivity of Leishmania strains in the outcome of visceral leishmaniasis. METHODS: In this study we have characterized four strains of L. infantum in terms of molecular typing, in vitro cultivation and differentiation. Two strains were isolated from HIV+ patients with visceral leishmaniasis (Bibiano and E390M), one strain was isolated from a cutaneous lesion in an immunocompetent patient (HL) and another internal reference strain causative of visceral leishmaniasis (ST) also from an immunocompetent patient was used for comparison. For this objective, we have compared their virulence by in vitro and in vivo infectivity in a murine model of visceral leishmaniasis. RESULTS: Molecular typing unraveled a new k26 sequence attributed to MON-284 zymodeme and allowed the generation of a molecular signature for the identification of each strain. In vitro cultivation enabled the production of promastigotes with comparable growth curves and metacyclogenesis development. The HL strain was the most infective, showing the highest parasite loads in vitro that were corroborated with the in vivo assays, 6 weeks post-infection in BALB/c mice. The two strains isolated from HIV+ patients, both belonging to two different zymodemes, revealed different kinetics of infection. CONCLUSION: Differences in in vitro and in vivo infectivity found in the murine model were then attributed to intrinsic characteristics of each strain. This work is supported by other studies that present the parasite's inherent features as factors for the multiplicity of clinical manifestations and severity of leishmaniasis.
eng
Leishmania infantum
Clinical isolates
Visceral leishmaniasis
Molecular typing
Metacyclogenesis
Infectivity
Tropism
Characterization of the biology and infectivity of Leishmania infantum viscerotropic and dermotropic strains isolated from HIV+ and HIV- patients in the murine model of visceral leishmaniasis
research article
URL
https://repisalud.isciii.es/bitstreams/ce432ead-e167-414e-8de7-b982a5ef284b/download
File
MD5
4e0049bad820660ca1efe83bada04da2
984664
application/pdf
CharacterizationOfTheBiology_2013.pdf
URL
https://repisalud.isciii.es/bitstreams/a3be517c-42c4-4a18-afa8-c417d0bc56e0/download
File
MD5
4d0b5a1db1817fb37623c57b6cd49a17
64416
text/plain
13071_2013_Article_913.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/a7c66bb2-ac01-44c1-abbf-19feee9c95a8/download
File
MD5
4d0b5a1db1817fb37623c57b6cd49a17
64416
text/plain
CharacterizationOfTheBiology_2013.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/79a86fb6-1d04-483b-bc3c-aa60b9919bba/download
File
MD5
336dc4138227aad9684a3fb7dd368816
64404
text/plain
CharacterizationOfTheBiology_2013.pdf.txt