2026-06-30T02:00:52Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/47922025-03-27T09:24:00Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Requena, Jose M
author
Chicharro, Carmen
author
García, Lineth
author
Parrado, Rudy
author
Puerta, Concepción J
author
Cañavate, Carmen
funder
Ministerio de Ciencia y Tecnología (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Agencia Española de Cooperación Internacional para el Desarrollo
funder
Fundación Ramón Areces
2017-09-04T16:25:07Z
2017-09-04T16:25:07Z
2012-04-28
Parasit Vectors. 2012; 5: 87
10.1186/1756-3305-5-87
1756-3305
Parasites & Vectors
22541251
http://hdl.handle.net/20.500.12105/4792
Background: The Leishmaniases are a group of clinically diverse diseases caused by parasites of the genus Leishmania. To distinguish between species is crucial for correct diagnosis and prognosis as well as for treatment decisions. Recently, sequencing of the HSP70 coding region has been applied in phylogenetic studies and for identifying of Leishmania species with excellent results. Methods: In the present study, we analyzed the 3'-untranslated region (UTR) of Leishmania HSP70-type I gene from 24 strains representing eleven Leishmania species in the belief that this non-coding region would have a better discriminatory capacity for species typing than coding regions. Results: It was observed that there was a remarkable degree of sequence conservation in this region, even between species of the subgenus Leishmania and Viannia. In addition, the presence of many microsatellites was a common feature of the 3'-UTR of HSP70-I genes in the Leishmania genus. Finally, we constructed dendrograms based on global sequence alignments of the analyzed Leishmania species and strains, the results indicated that this particular region of HSP70 genes might be useful for species (or species complex) typing, improving for particular species the discrimination capacity of phylogenetic trees based on HSP70 coding sequences. Given the large size variation of the analyzed region between the Leishmania and Viannia subgenera, direct visualization of the PCR amplification product would allow discrimination between subgenera, and a HaeIII-PCR-RFLP analysis might be used for differentiating some species within each subgenera. Conclusions: Sequence and phylogenetic analyses indicated that this region, which is readily amplified using a single pair of primers from both Old and New World Leishmania species, might be useful as a molecular marker for species discrimination.
eng
Leishmania
HSP70
3’UTR
Sequence analysis
Microsatellites
Phylogenetic analysis
Sequence analysis of the 3’-untranslated region of HSP70 (type I) genes in the genus Leishmania: its usefulness as a molecular marker for species identification
research article
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SequenceanalysisOfThe_2012.pdf
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13071_2012_Article_617.pdf.txt
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SequenceanalysisOfThe_2012.pdf.txt
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https://repisalud.isciii.es/bitstreams/a1689659-54db-40c6-ba2f-99a7c3c7ca48/download
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SequenceanalysisOfThe_2012.pdf.txt