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oai:repisalud.isciii.es:20.500.12105/277262026-09-25T14:02:00Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Sadaow, Lakkhana author Rodpai, Rutchanee author Smout, Michael J author Nakajima, Rie author Boonroumkaew, Patcharaporn author Sotillo, Javier author Tedla, Bemnet A author Luvira, Vor author Kitkhuandee, Amnat author Paonariang, Krisada author Sukeepaisarnjaroen, Wattana author Yamasaki, Hiroshi author Suttiprapa, Sutas author Laha, Thewarach author Sripa, Banchob author de Assis, Rafael author Jain, Aarti author Ittiprasert, Wannaporn author Mann, Victoria H author Wong, Yide author Felgner, Philip L author Maleewong, Wanchai author Brindley, Paul J author Loukas, Alex author Intapan, Pewpan M author 2025-07-01 Sensitive diagnostics are needed to improve management and surveillance of opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma (CCA) throughout East Asia. Herein we generate and screen an Opisthorchis viverrini recombinant secreted proteome to identity antibody biomarkers of liver fluke infection and CCA with sera from study participants in endemic populations and evaluate their utility as point-of-care immunochromatographic tests (PoC-ICTs). We incorporate two of the most promising antigens from the proteome array screen, P1 and P9, into PoC-ICTs to further validate their diagnostic performance. The P9-IgG4 PoC-ICT is superior amongst the single recombinant antigen tests for diagnosing fluke infection as well as fluke-induced CCA, and out-performs parasite crude extract-IgG ICTs. Here we identify two biomarkers of O. viverrini infection and infection-associated CCA that could form the basis of novel antibody serodiagnostic tests for human liver fluke infection and associated cancer. Sadaow, L., Rodpai, R., Smout, M.J. et al. Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma. Nat Commun 16, 5965 (2025). https://doi.org/10.1038/s41467-025-61043-2. https://hdl.handle.net/20.500.12105/27726 40595543 10.1038/s41467-025-61043-2 Nature Communications Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.