2026-09-21T01:54:17Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/277212026-09-19T00:16:27Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609col_20.500.12105_19617col_20.500.12105_25179
Repisalud
author
Varea, Covadonga
author
Martin-Galiano, Antonio Javier
author
Vázquez-Ávila, Sara
author
Montero-Calle, Ana Maria
author
Cesteros, Julia
author
Perteguer-Prieto, Maria Jesus
author
Peláez-García, Alberto
author
Barderas Manchado, Rodrigo
author
García-Rodríguez, Juan José
author
Sotillo, Javier
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2026-09-18T10:27:51Z
2026-09-18T10:27:51Z
2026-08-26
Varea, C., Martín-Galiano, A.J., Vazquez, S. et al. Nippostrongylus brasiliensis and Heligmosomoides polygyrus exert anti-inflammatory effects through distinct immunomodulatory mechanisms in murine macrophages in vitro. Sci Rep (2026). https://doi.org/10.1038/s41598-026-67387-z
10.1038/s41598-026-67387-z
Scientific Reports
https://hdl.handle.net/20.500.12105/27721
Hookworm infections affect hundreds of millions of people worldwide and rank among the most significant neglected tropical diseases in terms of morbidity. Due to the inverse correlation between hookworm infection and the incidence of immune-mediated inflammatory diseases, considerable research has focused on understanding how parasitic helminths modulate host inflammation. Heligmosomoides polygyrus and Nippostrongylus brasiliensis are two hookworm-like rodent models widely used to investigate fundamental aspects of immune regulation. However, no comparative study has yet analysed the distinct immunological pathways activated by these helminths in their hosts. In this study, we compared cytokine profiles and M1/M2 macrophage polarization markers induced by H. polygyrus and N. brasiliensis, alongside proteomic pathways involved in their activation, using the RAW 264.7 murine macrophage cell line. H. polygyrus downregulated proinflammatory cytokines such as TNF-α and MCP-1, whereas N. brasiliensis did not affect TNF-α expression. Both parasites upregulated Th2 and regulatory cytokines, including IL-4 and TGF-β. Furthermore, H. polygyrus predominantly polarized macrophages toward an M2 phenotype, while N. brasiliensis maintained a balanced M1/M2 profile. Proteomic analysis revealed that N. brasiliensis and H. polygyrus exert their anti-inflammatory effects through distinct mechanisms, with N. brasiliensis acting via peripheral pathways and H. polygyrus via central regulatory mechanisms.
eng
Nippostrongylus brasiliensis
Heligmosomoides polygyrus
RAW264.7
Macrophages
Nematoda
Immunomodulation
Nippostrongylus brasiliensis and Heligmosomoides polygyrus exert anti-inflammatory effects through distinct immunomodulatory mechanisms in murine macrophages in vitro.
research article
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NippostrongylusBrasiliensisHeligmosomoidesPolygyrus_2026.pdf
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Supplementary_1_NippostrongylusBrasiliensisHeligmosomoidesPolygyrus_2026.pdf
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