2026-06-16T05:05:16Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/275342026-06-16T00:20:28Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Quero-Delgado, Marta author Codina Márquez, Helena author Gómez, Rafael author Terán, Francisco José author Muñoz-Fernández, M A author Jiménez, José Luis author Resino, Salvador author Sepulveda-Crespo, Daniel author Martinez, Isidoro author 2025-07-29 The dramatic surge in dengue cases in early 2024, endangering half the global population, urgently necessitates faster diagnostic methods. Nanotechnology-enabled optical biosensors offer a promising avenue, leveraging nanomaterial properties for highly sensitive detection of dengue virus (DENV), potentially surpassing conventional techniques in terms of simplicity, speed, and cost-effectiveness. This systematic review analyzes recent advancements in these biosensors for DENV diagnosis. Following PRISMA 2020 guidelines, we systematically searched PubMed, Embase, Scopus, Web of Science, and Cochrane Library databases (2010-June 1, 2025; OSF: https://osf.io/3gmey/). The methodological quality of the 98 included studies was assessed using a modified CASP checklist. Diverse nanotechnology-based optical biosensors were identified. SPR (36.7%) was the most common transducer, followed by fluorescence (24.5%), colorimetry (15.3%), and SERS (8.2%). Gold-based nanomaterials (35.7%) were most frequently employed, with silver nanomaterials (15.3%), quantum dots (15.3%), and graphene-based materials (15.3%) also showing promise. DENV NS1 protein was the primary target analyte (21.4%). Importantly, almost half of the studies (44.9%) used clinically relevant human samples. While many optical biosensors show promise, challenges hinder their demonstration of the true potential for point-of-care use in their current format; however, they offer high specificity and faster results, laying a strong foundation for cost-effective clinical diagnostics. Nanotechnology-driven optical biosensors offer a transformative approach for DENV detection. Advances in computational design and green synthesis of novel nanomaterials are key to addressing stability and field-deployment challenges. These innovations are crucial for developing robust, sensitive, and user-friendly tools to manage dengue and improve patient outcomes globally. Quero-Delgado, M., Codina, H., Gómez, R., Terán, F.J., Muñoz-Fernández, M.A., Jiménez, J.L., Resino, S., Sepúlveda-Crespo, D. and Martínez, I. (2026), Advances in Nanotechnology-Enabled Optical Biosensors for Dengue Virus Detection: A Systematic Review. Medicinal Research Reviews, 46: 70-111. https://doi.org/10.1002/med.70006 10.1002/med.70006 Medicinal Research Reviews 40734186 https://hdl.handle.net/20.500.12105/27534 Dengue virus Diagnostics Nanomaterials Optical biosensor Point‐of‐care Advances in Nanotechnology-Enabled Optical Biosensors for Dengue Virus Detection: A Systematic Review.