2026-06-16T05:04:18Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/275292026-06-16T00:20:20Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Treviño-Nakoura, Ana
author
Sepulveda-Crespo, Daniel
author
Bellon, José M
author
Codina, Helena
author
Amigot-Sánchez, Rafael
author
Quero-Delgado, Marta
author
Ryan, Pablo
author
Martinez, Isidoro
author
Resino, Salvador
funder
Instituto de Salud Carlos III
funder
Gilead Sciences (Spain)
funder
Centro de Investigación Biomédica en Red - CIBERINFEC (Enfermedades Infecciosas)
2026-06-15T10:50:32Z
2026-06-15T10:50:32Z
2024-10
Treviño-Nakoura A, Sepúlveda-Crespo D, Bellon JM, et al. Diagnostic performance of dried blood spot hepatitis C virus core antigen testing for hepatitis C screening: a systematic review and meta-analysis. J Med Virol. 2024;96:e70018. doi:10.1002/jmv.70018
10.1002/jmv.70018
Journal of Medical Virology
39428965
https://hdl.handle.net/20.500.12105/27529
Dried blood spot (DBS) sampling is increasingly used for hepatitis C virus (HCV) screening. HCVcAg testing offers a faster and more streamlined approach to diagnosing HCV infection. We conducted a systematic review and meta-analysis to assess the diagnostic performance of the Abbott ARCHITECT HCV Ag assay for screening active HCV infection using DBS samples. Eight studies (n = 1229) were selected among all published studies available up to October 4, 2024, in different databases with a search strategy registered (PROSPERO: CRD42022363975). The gold standard method was the HCV PCR test. Data were analyzed using the MIDAS module in STATA with a random effects model. Combined diagnostic accuracy measures were as follows: sensitivity 85%, specificity 100%, positive likelihood ratio (PLR) 233.1, negative likelihood ratio (NLR) 0.15, and summary receiver operating characteristic (SROC) 0.99. Likelihood ratios and Fagan's nomogram suggested that the HCVcAg assay with DBS samples can confirm or rule out active HCV infection with over 92% accuracy in high-prevalence settings (≥5%). However, in low-prevalence settings (≤1%), a confirmatory test must be required for positive results. The ability of the test to identify people without HCV infection was high regardless of HCV prevalence, with an error rate of less than 3%. This meta-analysis is subject to limitations, particularly due to the number of included studies and significant heterogeneity among them. HCV screening using the Abbott ARCHITECT HCV Ag assay with DBS samples showed excellent diagnostic performance, but its external validity may be limited when HCV prevalence is low (≤1%).
eng
HCV core antigen
Diagnostic performance
Dried blood spot
Hepatitis C
Screening
Diagnostic performance of dried blood spot hepatitis C virus core antigen testing for hepatitis C screening: A systematic review and meta-analysis.
review article
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