2026-06-14T23:37:23Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/273492026-03-26T01:20:51Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Soto-Heredero, Gonzalo author Gabandé-Rodríguez, Enrique author Carrasco, Elisa author Escrig-Larena, José Ignacio author Gómez de Las Heras, Manuel M author Delgado-Pulido, Sandra author Francos-Quijorna, Isaac author Blanco, Eva M author Fernández-Almeida, Álvaro author Abia, David author Rodríguez, María Josefa author Fernández-Díaz, Cristina M author Álvarez-Flores, María Beatriz author Ramírez de Molina, Ana author Jung, Sascha author Del Sol, Antonio author Zorita, Virginia author Sánchez-Cabo, Fátima author Torroja, Carlos author Mittelbrunn, María author 2025-05 Recent studies using single-cell RNA sequencing technology have uncovered several subpopulations of CD4 T cells that accumulate with aging. These age-associated T cells are emerging as relevant players in the onset of inflammaging and tissue senescence. Here, based on information provided by single-cell RNA sequencing data, we present a flow cytometry panel that allows the identification of age-associated T cell subsets in systematic larger analysis in mice. We use this panel to evaluate at the single-cell level mitochondrial and senescence marks in the different age-associated CD4 T cell subpopulations. Our analysis identifies a subpopulation of regulatory T (T) cells that is characterized by the extracellular expression of the co-inhibitory molecule killer cell lectin-like receptor subfamily G member 1 (KLRG1) and accumulates with aging in humans and mice. KLRG1-expressing T cells display senescence features such as mitochondrial alterations, increased expression of cell-cycle regulators and genomic DNA damage. Functionally, KLRG1 T cells show a reduced suppressive activity in vivo accompanied by a pro-inflammatory phenotype. Nat Aging. 2025 May;5(5):799-815. Nature Aging 40307497 https://hdl.handle.net/20.500.12105/27349 KLRG1 identifies regulatory T cells with mitochondrial alterations that accumulate with aging.