2026-05-14T16:37:46Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/273162026-03-17T01:15:56Zcom_20.500.12105_19586com_20.500.12105_2202col_20.500.12105_19587
Repisalud
author
Sendra, Miquel
author
McDole, Katie
author
de Dios Hourcade, Juan
author
Temiño, Susana
author
Raiola, Morena
author
Guignard, Léo
author
Keller, Philipp J
author
Domínguez, Jorge N
author
Torres, Miguel
funder
Fundación La Caixa
funder
Comunidad de Madrid (España)
2026-03-16T12:11:07Z
2026-03-16T12:11:07Z
2025-09-22
Dev Cell. 2025 Sep 22;60(18):2434-2444.e5.
Developmental Cell
40436021
https://hdl.handle.net/20.500.12105/27316
The formation of the primitive heart tube from cardiomyocytes and endocardial cells is a key event in mammalian development. Previous studies suggested that cardiomyocytes and endocardial cells segregate from a shared cardiac progenitor around the onset of gastrulation, yet their lineage relationship with other mesodermal tissues remains unclear. Using retrospective and prospective clonal analyses in mouse embryos, we traced cardiomyocyte and endocardial progenitors from the primitive streak to the heart tube. Our results identify two independent mesodermal populations specified around gastrulation onset. While each of these populations is unipotent in producing cardiomyocytes or endocardium, they retain multipotency and contribute to different subsets of non-cardiac mesoderm. Nonetheless, live imaging identifies simultaneous ingression and intermingling of these two mesodermal lineages in the primitive streak, showing their coordinated specification and migration. The proposed model for cardiac progenitor specification will help understanding the origins of congenital heart diseases and designing tissue engineering strategies.
eng
cardiac progenitors
cardiomyocytes
cell fate
clonal analysis
endocardial cells
heart development
lineage specification
lineage tracing
live imaging
mesoderm
Myocardium and endocardium of the early mammalian heart tube arise from independent multipotent lineages specified at the primitive streak.
research article
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URL
https://repisalud.isciii.es/bitstreams/8cd073ff-5ad7-4786-bfaa-d18c0c2fac7f/download
File
MD5
12a159c8a5f4d95cb55e94e9c53e9ea1
10710555
application/pdf
Myocardium and endocardium_Dev Cell_2025.pdf