2026-05-14T16:37:46Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/273162026-03-17T01:15:56Zcom_20.500.12105_19586com_20.500.12105_2202col_20.500.12105_19587

00925njm 22002777a 4500 dc Sendra, Miquel author McDole, Katie author de Dios Hourcade, Juan author Temiño, Susana author Raiola, Morena author Guignard, Léo author Keller, Philipp J author Domínguez, Jorge N author Torres, Miguel author 2025-09-22 The formation of the primitive heart tube from cardiomyocytes and endocardial cells is a key event in mammalian development. Previous studies suggested that cardiomyocytes and endocardial cells segregate from a shared cardiac progenitor around the onset of gastrulation, yet their lineage relationship with other mesodermal tissues remains unclear. Using retrospective and prospective clonal analyses in mouse embryos, we traced cardiomyocyte and endocardial progenitors from the primitive streak to the heart tube. Our results identify two independent mesodermal populations specified around gastrulation onset. While each of these populations is unipotent in producing cardiomyocytes or endocardium, they retain multipotency and contribute to different subsets of non-cardiac mesoderm. Nonetheless, live imaging identifies simultaneous ingression and intermingling of these two mesodermal lineages in the primitive streak, showing their coordinated specification and migration. The proposed model for cardiac progenitor specification will help understanding the origins of congenital heart diseases and designing tissue engineering strategies. Dev Cell. 2025 Sep 22;60(18):2434-2444.e5. Developmental Cell 40436021 https://hdl.handle.net/20.500.12105/27316 cardiac progenitors cardiomyocytes cell fate clonal analysis endocardial cells heart development lineage specification lineage tracing live imaging mesoderm Myocardium and endocardium of the early mammalian heart tube arise from independent multipotent lineages specified at the primitive streak.