2026-04-29T20:47:18Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/272512026-02-22T01:18:31Zcom_20.500.12105_2327com_20.500.12105_2290col_20.500.12105_16991
Repisalud
author
Hyenne, Vincent
author
Ghoroghi, Shima
author
Collot, Mayeul
author
Bons, Joanna
author
Follain, Gautier
author
Harlepp, Sébastien
author
Mary, Benjamin
author
Bauer, Jack
author
Mercier, Luc
author
Busnelli, Ignacio
author
Lefebvre, Olivier
author
Fekonja, Nina
author
Garcia-Leon, Maria J
author
Machado, Pedro
author
Delalande, François
author
López, Ana Amor
author
Silva, Susana Garcia
author
Verweij, Frederik J
author
van Niel, Guillaume
author
Djouad, Farida
author
Peinado, Héctor
author
Carapito, Christine
author
Klymchenko, Andrey S
author
Goetz, Jacky G
2026-02-21T16:36:20Z
2026-02-21T16:36:20Z
2019-02-25
30745140
https://hdl.handle.net/20.500.12105/27251
Tumor extracellular vesicles (EVs) mediate the communication between tumor and stromal cells mostly to the benefit of tumor progression. Notably, tumor EVs travel in the bloodstream, reach distant organs, and locally modify the microenvironment. However, visualizing these events in vivo still faces major hurdles. Here, we describe an approach for tracking circulating tumor EVs in a living organism: we combine chemical and genetically encoded probes with the zebrafish embryo as an animal model. We provide a first description of tumor EVs' hemodynamic behavior and document their intravascular arrest. We show that circulating tumor EVs are rapidly taken up by endothelial cells and blood patrolling macrophages and subsequently stored in degradative compartments. Finally, we demonstrate that tumor EVs activate macrophages and promote metastatic outgrowth. Overall, our study proves the usefulness and prospects of zebrafish embryo to track tumor EVs and dissect their role in metastatic niches formation in vivo.
eng
correlated light and electron microscopy
exosomes
extracellular vesicles
patrolling macrophages
premetastatic niche
zebrafish
Studying the Fate of Tumor Extracellular Vesicles at High Spatiotemporal Resolution Using the Zebrafish Embryo.
research article
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