2026-04-29T20:47:16Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/272512026-02-22T01:18:31Zcom_20.500.12105_2327com_20.500.12105_2290col_20.500.12105_16991

00925njm 22002777a 4500 dc Hyenne, Vincent author Ghoroghi, Shima author Collot, Mayeul author Bons, Joanna author Follain, Gautier author Harlepp, Sébastien author Mary, Benjamin author Bauer, Jack author Mercier, Luc author Busnelli, Ignacio author Lefebvre, Olivier author Fekonja, Nina author Garcia-Leon, Maria J author Machado, Pedro author Delalande, François author López, Ana Amor author Silva, Susana Garcia author Verweij, Frederik J author van Niel, Guillaume author Djouad, Farida author Peinado, Héctor author Carapito, Christine author Klymchenko, Andrey S author Goetz, Jacky G author 2019-02-25 Tumor extracellular vesicles (EVs) mediate the communication between tumor and stromal cells mostly to the benefit of tumor progression. Notably, tumor EVs travel in the bloodstream, reach distant organs, and locally modify the microenvironment. However, visualizing these events in vivo still faces major hurdles. Here, we describe an approach for tracking circulating tumor EVs in a living organism: we combine chemical and genetically encoded probes with the zebrafish embryo as an animal model. We provide a first description of tumor EVs' hemodynamic behavior and document their intravascular arrest. We show that circulating tumor EVs are rapidly taken up by endothelial cells and blood patrolling macrophages and subsequently stored in degradative compartments. Finally, we demonstrate that tumor EVs activate macrophages and promote metastatic outgrowth. Overall, our study proves the usefulness and prospects of zebrafish embryo to track tumor EVs and dissect their role in metastatic niches formation in vivo. 30745140 https://hdl.handle.net/20.500.12105/27251 correlated light and electron microscopy exosomes extracellular vesicles patrolling macrophages premetastatic niche zebrafish Studying the Fate of Tumor Extracellular Vesicles at High Spatiotemporal Resolution Using the Zebrafish Embryo.