2026-08-29T12:40:21Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/272402026-02-19T01:20:06Zcom_20.500.12105_19586com_20.500.12105_2202col_20.500.12105_19587
Repisalud
author
Martínez-M, J A
author
Minguet, S
author
Gonzalo, P
author
Soro, P G
author
de Andrés, B
author
Izcue, A
author
Marcos, M A
author
Gaspar, M L
2026-02-18T14:29:09Z
2026-02-18T14:29:09Z
2001-09-15
Blood. 2001 Sep 15;98(6):1862-71.
Blood
11535523
https://hdl.handle.net/20.500.12105/27240
Lymphohematopoietic progenitors derived from midgestation mouse embryos were established in long-term cultures with stromal cell monolayers and interleukin 7 (IL-7), giving rise to B-lineage cell lines. The initial emergence and in vitro establishment of these early embryo cell lines were highly sensitive to IL-7-mediated signals, in comparison to cell lines similarly obtained using precursors from late fetal liver (> 13 days postcoitum) and adult bone marrow. The early embryo-derived progenitors spontaneously differentiated in vitro to CD19(+)IgM(+) immature B cells in the presence of optimal concentrations of IL-7, in contrast to those progenitors obtained from late gestation and adult mice, whose differentiation only occurred in the absence of IL-7. The newly in vitro-generated B cells of the early embryo cell lines repopulated adult immunodeficient severe combined immunodeficient mice on their adoptive transfer in vivo and generated specific humoral immune responses after immunization.
eng
Long-lived polyclonal B-cell lines derived from midgestation mouse embryo lymphohematopoietic progenitors reconstitute adult immunodeficient mice.
research article
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URL
https://repisalud.isciii.es/bitstreams/6fb2f800-f2b1-4fd5-b9ca-5a0fed4614e6/download
File
MD5
4d9ca86dfc010d03645dcf83dbe67694
354118
application/pdf
Long-lived polyclonal B-cell_Blood_2001.pdf