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00925njm 22002777a 4500 dc Valderrama, M J author de Silóniz, M I author Gonzalo, P author Peinado, J M author 1999-02 A selective and differential solid medium, called Kluyveromyces Differential Medium (KDM), is described for the isolation of Kluyveromyces marxianus and K. lactis from dairy products. Its discriminative potential is based on the detection of the enzyme beta-galactosidase, in the absence of lactose. Of the more than 95 strains tested, including yeasts, bacteria, and filamentous fungus, only the strains of K. marxianus and K. lactis produced blue colonies on the medium due to the presence of X-Gal/ IPTG. The bacterial strains were not able to grow in KDM. On this basis, the medium was very satisfactory when testing naturally or experimentally contaminated dairy food products. When quality assessment tests were performed, optimal values of productivity (growth and color) and selectivity were obtained for K. marxianus and K. lactis. J Food Prot. 1999 Feb;62(2):189-93. Journal of Food Protection 10030640 https://hdl.handle.net/20.500.12105/27239 A differential medium for the isolation of Kluyveromyces marxianus and Kluyveromyces lactis from dairy products.