2026-04-29T20:47:12Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/272282026-02-13T01:20:18Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Zubiri, Irene
author
Posada-Ayala, Maria
author
Sanz-Maroto, Aroa
author
Calvo, Enrique
author
Martin-Lorenzo, Marta
author
Gonzalez-Calero, Laura
author
de la Cuesta, Fernando
author
Lopez, Juan A
author
Fernandez-Fernandez, Beatriz
author
Ortiz, Alberto
author
Vivanco, Fernando
author
Alvarez-Llamas, Gloria
2026-02-12T17:10:02Z
2026-02-12T17:10:02Z
2014-01-16
J Proteomics. 2014 Jan 16:96:92-102.
Journal of Proteomics
24211404
https://hdl.handle.net/20.500.12105/27228
Diabetic nephropathy (DN) is a major complication of diabetes mellitus (DM), the most frequent cause of end-stage renal disease (ESRD). Exosomes isolated from urine are considered a rich non-invasive source of markers for renal events. Proteinuria associated with DN patients at advanced stages may result in "contamination" of exosomal fraction by co-precipitation of high abundance urine proteins, making it enormously difficult to obtain a reliable comparison of healthy individuals and DN patients and to detect minor proteins. We evaluated different protocols for urinary exosome isolation (ultracentrifugation-based and Exoquick® reagent-based) in combination with an easy and quick depletion procedure of contaminating high abundance proteins (albumin). The optimal methodology was then applied to investigate the proteome of human urinary exosomes in DN and controls using spectral counting LC-MS/MS analysis followed by selected reaction monitoring (SRM) confirmation. A panel of 3 proteins (AMBP, MLL3, and VDAC1) is differentially present in urinary exosomes from DN patients, opening a new field of research focused on improving diagnosis and follow-up of this pathology.
eng
Depletion
Diabetic nephropathy
Exosomes
Label-free quantitation
Proteomics
Selected reaction monitoring
Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis.
research article
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URL
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Diabetic nephropathy induces changes_J Proteomics_2014 .pdf