2026-08-29T19:28:20Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/272262026-02-12T01:21:13Zcom_20.500.12105_19586com_20.500.12105_2202col_20.500.12105_19587
Repisalud
author
Castillo, Luis
author
Calvo, Enrique
author
Martínez, Ana I
author
Ruiz-Herrera, José
author
Valentín, Eulogio
author
Lopez, Juan A
author
Sentandreu, Rafael
2026-02-11T16:52:03Z
2026-02-11T16:52:03Z
2008-09
Proteomics. 2008 Sep;8(18):3871-81.
Proteomics
18712765
https://hdl.handle.net/20.500.12105/27226
Considering the importance of proteins in the structure and function of the cell wall of Candida albicans, we analyzed the cell wall subproteome of this important human pathogen by LC coupled to MS (LC-MS) using different protein extraction procedures. The analyzed samples included material extracted by hydrogen fluoride-pyridine (HF-pyridine), and whole SDS-extracted cell walls. The use of this latter innovative procedure gave similar data as compared to the analysis of HF-pyridine extracted proteins. A total of 21 cell wall proteins predicted to contain a signal peptide were identified, together with a high content of potentially glycosylated Ser/Thr residues, and the presence of a GPI motif in 19 of them. We also identified 66 "atypical" cell wall proteins that lack the above-mentioned characteristics. After tryptic removal of the most accessible proteins in the cell wall, several of the same expected GPI proteins and the most commonly found "atypical" wall proteins were identified. This result suggests that proteins are located not only at the cell wall surface, but are embedded within the cell wall itself. These results, which include new identified cell wall proteins, and comparison of proteins in blastospore and mycelial walls, will help to elucidate the C. albicans cell wall architecture.
eng
A study of the Candida albicans cell wall proteome.
research article
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URL
https://repisalud.isciii.es/bitstreams/3e321bcf-9f97-45ee-9c14-a10add235658/download
File
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7ffb4165bba678359c8d414c250bfbda
261728
application/pdf
A study of the Candida albicans_Proteomics_2008.pdf