2026-04-29T07:25:10Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/271502026-01-26T20:27:15Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19608

00925njm 22002777a 4500 dc Virseda-Berdices, Ana author Brochado-Kith, Oscar author Díez, Cristina author Hontañon, Victor author Berenguer, Juan author González-García, Juan author Rojo, David author Fernandez-Rodriguez, Amanda author Ibañez-Samaniego, Luis author Llop-Herrera, Elba author Olveira, Antonio author Perez-Latorre, Leire author Barbas, Coral author Rava, Marta author Resino, Salvador author Jimenez-Sousa, Maria Angeles author CoRIS author 2022-02-23 Background: Patients with a significant decrease in hepatic venous pressure gradient (HVPG) have a considerable reduction of liver complications and higher survival after HCV eradication. Objectives: To evaluate the association between the baseline blood microbiome and the changes in HVPG after successful direct-acting antiviral (DAA) therapy in patients with HCV-related cirrhosis. Methods: We performed a prospective study in 32 cirrhotic patients (21 HIV positive) with clinically significant portal hypertension (HVPG ≥10 mmHg). Patients were assessed at baseline and 48 weeks after HCV treatment completion. The clinical endpoint was a decrease in HVPG of ≥20% or HVPG <12 mmHg at the end of follow-up. Bacterial 16S ribosomal DNA was sequenced using MiSeq Illumina technology, inflammatory plasma biomarkers were investigated using ProcartaPlex immunoassays and the metabolome was investigated using GC-MS. Results: During the follow-up, 47% of patients reached the clinical endpoint. At baseline, those patients had a higher relative abundance of Corynebacteriales and Diplorickettsiales order, Diplorickettsiaceae family, Corynebacterium and Aquicella genus and Undibacterium parvum species organisms and a lower relative abundance of Oceanospirillales and Rhodospirillales order, Halomonadaceae family and Massilia genus organisms compared with those who did not achieve the clinical endpoint according to the LEfSe algorithm. Corynebacteriales and Massilia were consistently found within the 10 bacterial taxa with the highest differential abundance between groups. Additionally, the relative abundance of the Corynebacteriales order was inversely correlated with IFN-γ, IL-17A and TNF-α levels and the Massilia genus with glycerol and lauric acid. Conclusions: Baseline-specific bacterial taxa are related to an HVPG decrease in patients with HCV-related cirrhosis after successful DAA therapy. Ana Virseda-Berdices, Oscar Brochado-Kith, Cristina Díez, Victor Hontañon, Juan Berenguer, Juan González-García, David Rojo, Amanda Fernández-Rodríguez, Luis Ibañez-Samaniego, Elba Llop-Herrera, Antonio Olveira, Leire Perez-Latorre, Coral Barbas, Marta Rava, Salvador Resino, María Angeles Jiménez-Sousa, on behalf of the ESCORIAL Study Group, Blood microbiome is associated with changes in portal hypertension after successful direct-acting antiviral therapy in patients with HCV-related cirrhosis, Journal of Antimicrobial Chemotherapy, Volume 77, Issue 3, March 2022, Pages 719–726, https://doi.org/10.1093/jac/dkab444. https://hdl.handle.net/20.500.12105/27150 34888660 10.1093/jac/dkab444 Journal of Antimicrobial Chemotherapy Blood microbiome is associated with changes in portal hypertension after successful direct-acting antiviral therapy in patients with HCV-related cirrhosis.