2026-05-20T19:10:30Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/271382026-01-13T01:18:39Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Torres Garcia, Ana Maria
author
Montero-Calle, Ana Maria
author
Lozano-Rendal, Marina
author
Sanchez Herrero, Carmen
author
Bernardo, Lorena
author
Solana, Jose Carlos
author
San Martin, Juan Victor
author
Barderas Manchado, Rodrigo
author
Moreno, Javier
author
Carrillo, Eugenia
2026-01-12T13:26:38Z
2026-01-12T13:26:38Z
2025
Torres A, Montero-Calle A, Lozano-Rendal M, Sánchez C, Bernardo L, Solana JC, San Martin JV, Barderas R, Moreno J and Carrillo E (2025) Proteomics of plasma-derived extracellular vesicles from human patients identifies biomarkers for monitoring visceral leishmaniasis therapy. Front. Immunol. 16:1646335. doi: 10.3389/fimmu.2025.1646335.
10.3389/fimmu.2025.1646335
Frontiers in Immunology
41019078
https://hdl.handle.net/20.500.12105/27138
Introduction: The most severe form of leishmaniasis, visceral leishmaniasis (VL), lacks standardized validated early predictors of treatment success or relapse. To distinguish between active infection and successful treatment, we searched for protein biomarkers in plasma-derived extracellular vesicles (EVs). Methods: The proteomic profiles of EVs from immunocompetent patients with active VL (n=12) or 1, 3, or 6 months after completing a standard treatment regimen (n=12 each) were analyzed by LC-MS/MS. Six candidate biomarkers were further tested by ELISA in whole plasma. Results: 132 human proteins were differentially expressed in active VL- versus successfully treated patients. Pathway analysis identified pathogenic mechanisms associated with VL and pathways related to effective cure. SAA is directly measurable in whole plasma and exhibits differential expression levels, emerging as a promising, easily measurable, non-specific prognostic biomarker for patient management. Remarkably, we also identified Leishmania spp. proteins in EV samples, indicating a new source of parasite biomarkers in human samples. Conclusion: Plasma EVs contain protein biomarkers that can be used to monitor the response to treatment, some of which are detectable in whole plasma after 1 month of treatment. Our study also provides a proteomic landscape of plasma EVs involved in VL, offering insight into the pathogenesis of this complex disease.
eng
Biomarkers
Cured patients
Extracellular vesicles
Plasma
Proteomics
Visceral leishmaniasis
Proteomics of plasma-derived extracellular vesicles from human patients identifies biomarkers for monitoring visceral leishmaniasis therapy.
research article
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ProteomicsPlasmaDerivedExtracellular_2025.pdf
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Supplementary_ProteomicsPlasmaDerivedExtracellular_2025.zip