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oai:repisalud.isciii.es:20.500.12105/271262026-01-11T01:19:45Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Torres-Cano, Alba author de Armentia, Cristina author Roldán, Alejandra author López-Peralta, Elena author Manosalva, Juliana author Merino-Amador, Paloma author González-Romo, Fernando author Puig-Asensio, Mireia author Ardanuy, Carmen author Martín-Gómez, María Teresa author Romero-Herrero, Daniel author Pérez-Ayala, Ana author López-Lomba, Marta author Durán-Valle, María Teresa author Sánchez-Romero, Isabel author Muñoz-Algarra, María author Roiz-Mesones, María Pía author Lara-Plaza, Isabel author Ruíz Pérez de Pipaón, Maite author Megías-Lobón, Gregoria author Mantecón-Vallejo, María Ángeles author Alcazar-Fuoli, Laura author Megias Vazquez, Diego author Zaragoza, Oscar author 2025-04-09 In recent years, there has been an increase in the incidence of fluconazole-non-susceptible (FNS) . The reasons why these strains are able to colonize hospitals remain unknown. It is also unclear whether these strains exhibit resistance to the disinfectants used in hospitals, facilitating their spread. For these reasons, in this work, we aimed to investigate whether fluconazole resistance was associated with virulence traits and the resistance of these strains to common hospital disinfectants. The general conclusion of the study was that more than 95% of the FNS strains, regardless of the resistance mutation they carried, had filamentation problems, whereas around 75% of the susceptible strains formed pseudohyphae and were capable of filamentation. This 95% of the FNS strains did not form pseudohyphae, did not invade agar, and did not form biofilms, while the susceptible strains exhibited the opposite behaviour. Through microfluidics experiments, we observed that both the susceptible and FNS strains were capable of adhering to a plastic surface under dynamic conditions, but the FNS strains formed unstable aggregates that did not remain attached to the surface, confirming the filamentation defect of these strains. In the second part of the study, we observed that FNS strains are susceptible to clinical disinfectants, although they presented a slight resistance to some of them, such as chlorhexidine, compared to susceptible isolates. In this work, we address important aspects to understand the dissemination of FNS strains in clinical outbreaks. Torres-Cano A, de Armentia C, Roldán A, López-Peralta E, Manosalva J, Merino-Amador P, González-Romo F, Puig-Asensio M, Ardanuy C, Martín-Gómez MT, Romero-Herrero D, Pérez-Ayala A, López-Lomba M, Durán-Valle MT, Sánchez-Romero I, Muñoz-Algarra M, Roiz-Mesones MP, Lara-Plaza I, Ruíz Pérez de Pipaón M, Megías-Lobón G, Mantecón-Vallejo MÁ, Alcázar-Fuoli L, Megías D, Zaragoza O. Resistance to Azoles in Candida parapsilosis Isolates from Spain Is Associated with an Impairment in Filamentation and Biofilm Formation. J Fungi (Basel). 2025 Apr 9;11(4):299. doi: 10.3390/jof11040299. PMID: 40278120; PMCID: PMC12028211. 10.3390/jof11040299 Journal of Fungi 40278120 https://hdl.handle.net/20.500.12105/27126 Candida parapsilosis Adhesion Antifungal Azole resistance Biofilm Disinfectant Pseudohyphae Resistance to Azoles in Isolates from Spain Is Associated with an Impairment in Filamentation and Biofilm Formation.