2026-05-21T23:11:31Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/271252026-01-11T01:21:19Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Rossi, Suelen Andreia
author
Garcia-Barbazan, Irene
author
Chamorro-Herrero, Irene
author
Taborda, Carlos Pelleschi
author
Zaragoza, Oscar
author
Zambrano, Alberto
2026-01-11T00:27:31Z
2026-01-11T00:27:31Z
2024
Rossi SA, García-Barbazán I, Chamorro-Herrero I, Taborda CP, Zaragoza Ó, Zambrano A. Use of 2D minilungs from human embryonic stem cells to study the interaction of Cryptococcus neoformans with the respiratory tract. Microbes Infect. 2024 Mar-Apr;26(3):105260. doi: 10.1016/j.micinf.2023.105260. Epub 2023 Nov 18. PMID: 37981028.
10.1016/j.micinf.2023.105260
Microbes and Infection
37981028
https://hdl.handle.net/20.500.12105/27125
Organoids can meet the needs between the use of cell culture and in vivo work, bringing together aspects of multicellular tissues, providing a more similar in vitro system for the study of various components, including host-interactions with pathogens and drug response. Organoids are structures that resemble organs in vivo, originating from pluripotent stem cells (PSCs) or adult stem cells (ASCs). There is great interest in deepening the understanding of the use of this technology to produce information about fungal infections and their treatments. This work aims the use 2D human lung organoid derived from human embryonic stem cells (hESCs), to investigate Cryptococcus neoformans-host interactions. C. neoformans is an opportunistic fungus acquired by inhalation that causes systemic mycosis mainly in immunocompromised individuals. Our work highlights the suitability of human minilungs for the study of C. neoformans infection (adhesion, invasion and replication), the interaction with the surfactant and induction of the host's alveolar pro-inflammatory response.
eng
Alveolar cells
Biosurfactants
Cryptococcus neoformans
Disease modeling
Fungal–pathogen interaction
Human embryonic stem cells
Human organoids
Human pluripotent stem cells
Minilungs
Surfactant proteins
hESCs
Use of 2D minilungs from human embryonic stem cells to study the interaction of Cryptococcus neoformans with the respiratory tract.
research article
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https://repisalud.isciii.es/bitstreams/8b53e5e5-8b2e-4da4-8940-5484845762df/download
File
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Use2DMinilungsHuman_2024.pdf
URL
https://repisalud.isciii.es/bitstreams/ca37cc70-3445-4d83-b4f4-c2dfe9fd7525/download
File
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Supplementary_Use2DMinilungsHuman_2024.pdf