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oai:repisalud.isciii.es:20.500.12105/271252026-01-11T01:21:19Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Rossi, Suelen Andreia author Garcia-Barbazan, Irene author Chamorro-Herrero, Irene author Taborda, Carlos Pelleschi author Zaragoza, Oscar author Zambrano, Alberto author 2024 Organoids can meet the needs between the use of cell culture and in vivo work, bringing together aspects of multicellular tissues, providing a more similar in vitro system for the study of various components, including host-interactions with pathogens and drug response. Organoids are structures that resemble organs in vivo, originating from pluripotent stem cells (PSCs) or adult stem cells (ASCs). There is great interest in deepening the understanding of the use of this technology to produce information about fungal infections and their treatments. This work aims the use 2D human lung organoid derived from human embryonic stem cells (hESCs), to investigate Cryptococcus neoformans-host interactions. C. neoformans is an opportunistic fungus acquired by inhalation that causes systemic mycosis mainly in immunocompromised individuals. Our work highlights the suitability of human minilungs for the study of C. neoformans infection (adhesion, invasion and replication), the interaction with the surfactant and induction of the host's alveolar pro-inflammatory response. Rossi SA, García-Barbazán I, Chamorro-Herrero I, Taborda CP, Zaragoza Ó, Zambrano A. Use of 2D minilungs from human embryonic stem cells to study the interaction of Cryptococcus neoformans with the respiratory tract. Microbes Infect. 2024 Mar-Apr;26(3):105260. doi: 10.1016/j.micinf.2023.105260. Epub 2023 Nov 18. PMID: 37981028. 10.1016/j.micinf.2023.105260 Microbes and Infection 37981028 https://hdl.handle.net/20.500.12105/27125 Alveolar cells Biosurfactants Cryptococcus neoformans Disease modeling Fungal–pathogen interaction Human embryonic stem cells Human organoids Human pluripotent stem cells Minilungs Surfactant proteins hESCs Use of 2D minilungs from human embryonic stem cells to study the interaction of Cryptococcus neoformans with the respiratory tract.