2026-04-29T04:10:08Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/271232026-01-11T01:16:41Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Valdezate, Sylvia
author
Medina-Pascual, Maria Jose
author
Rodríguez-García, César
author
Perez-Vazquez, Maria
author
Valiente, Mónica
author
Serrano, José
author
Rodríguez, Marta
author
De Toro, Inmaculada
author
Villalón, Pilar
2026-01-10T22:53:14Z
2026-01-10T22:53:14Z
2026-01
Valdezate S, Medina-Pascual MJ, Rodríguez-García C, Pérez-Vázquez M, Valiente M, Serrano J, Rodríguez M, De Toro I, Villalón P. Beyond the plate: Genomic characterization of an extensive school Clostridium perfringens type F outbreak. Int J Infect Dis. 2026 Jan;162:108179. doi: 10.1016/j.ijid.2025.108179. Epub 2025 Nov 4. PMID: 41197860.
https://hdl.handle.net/20.500.12105/27123
41197860
10.1016/j.ijid.2025.108179
International Journal of Infectious Diseases
Objectives: Clostridium perfringens is one of the leading causes of food-borne outbreaks (FBO). This study explores the epidemiological and genomic features of a major FBO occurred in a Spanish school in 2023. Methods: Through genomic analysis, the virulence factors and relatedness of C. perfringens strains involved in a widespread gastroenteritis outbreak were identified RESULTS: The FBO had a unimodal epidemic curve, affecting 526 people. The likely source was the meat in Bolognese sauce. The clinical strains, which belonged to two different clonal lineages (ST-804/cgST-804 and ST5/cgST-801), carried a wide virulence profile with multiple toxins including CPA, enterotoxin CPE, cytolytic toxin CPB2, perfringolysin, collagenase A, clostripain, hyaluronidases (NagHIJKL) and sialidases (NanIHJ). FBO strains of C. perfringens type F carry the cpe gene on the chromosome rather than on a plasmid. However, during this outbreak, the cpe gene, encoding the CPE enterotoxin, was mobilized by a pCPF4969-like plasmid. Susceptibility to metronidazole and vancomycin was retained. Distinct phage repertoires were observed in both lineages. Conclusions: This is the first genomic study of a C. perfringens FBO in Spain, suggesting a multiple contamination sources in the same outbreak. The findings underscore substantial public health concerns related to food safety practices within large-scale user facilities.
eng
Beta2 toxin
Clostridium perfringens, Type F
Food-borne diseases
Outbreak
Perfringolysin o
Plasmid enterotoxin
School
Beyond the plate: Genomic characterization of an extensive school Clostridium perfringens type F outbreak.
research article
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