2026-04-29T02:38:02Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/271232026-01-11T01:16:41Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Valdezate, Sylvia author Medina-Pascual, Maria Jose author Rodríguez-García, César author Perez-Vazquez, Maria author Valiente, Mónica author Serrano, José author Rodríguez, Marta author De Toro, Inmaculada author Villalón, Pilar author 2026-01 Objectives: Clostridium perfringens is one of the leading causes of food-borne outbreaks (FBO). This study explores the epidemiological and genomic features of a major FBO occurred in a Spanish school in 2023. Methods: Through genomic analysis, the virulence factors and relatedness of C. perfringens strains involved in a widespread gastroenteritis outbreak were identified RESULTS: The FBO had a unimodal epidemic curve, affecting 526 people. The likely source was the meat in Bolognese sauce. The clinical strains, which belonged to two different clonal lineages (ST-804/cgST-804 and ST5/cgST-801), carried a wide virulence profile with multiple toxins including CPA, enterotoxin CPE, cytolytic toxin CPB2, perfringolysin, collagenase A, clostripain, hyaluronidases (NagHIJKL) and sialidases (NanIHJ). FBO strains of C. perfringens type F carry the cpe gene on the chromosome rather than on a plasmid. However, during this outbreak, the cpe gene, encoding the CPE enterotoxin, was mobilized by a pCPF4969-like plasmid. Susceptibility to metronidazole and vancomycin was retained. Distinct phage repertoires were observed in both lineages. Conclusions: This is the first genomic study of a C. perfringens FBO in Spain, suggesting a multiple contamination sources in the same outbreak. The findings underscore substantial public health concerns related to food safety practices within large-scale user facilities. Valdezate S, Medina-Pascual MJ, Rodríguez-García C, Pérez-Vázquez M, Valiente M, Serrano J, Rodríguez M, De Toro I, Villalón P. Beyond the plate: Genomic characterization of an extensive school Clostridium perfringens type F outbreak. Int J Infect Dis. 2026 Jan;162:108179. doi: 10.1016/j.ijid.2025.108179. Epub 2025 Nov 4. PMID: 41197860. https://hdl.handle.net/20.500.12105/27123 41197860 10.1016/j.ijid.2025.108179 International Journal of Infectious Diseases Beta2 toxin Clostridium perfringens, Type F Food-borne diseases Outbreak Perfringolysin o Plasmid enterotoxin School Beyond the plate: Genomic characterization of an extensive school Clostridium perfringens type F outbreak.