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oai:repisalud.isciii.es:20.500.12105/270572025-12-17T01:20:11Zcom_20.500.12105_19586com_20.500.12105_2202col_20.500.12105_19587

00925njm 22002777a 4500 dc Marín-Vicente, Consuelo author Calvo, Enrique author Rodríguez, José Manuel author Villa Del Campo, Cristina author Sierra, Rocío author Végvári, Ákos author Zubarev, Roman A author Torres, Miguel author Vázquez, Jesús author 2024 Mass spectrometry-based proteomics has traditionally been limited by the amount of input material for analysis. Single-cell proteomics has emerged as a challenging discipline due to the ultra-high sensitivity required. Isobaric labeling-based multiplex strategies with a carrier proteome offer an approach to overcome the sensitivity limitations. Following this as the basic strategy, we show here the general workflow for preparing cells for single-cell mass spectrometry-based proteomics. This protocol can also be applied to manually isolated cells when large cells, such as cardiomyocytes, are difficult to isolate properly with conventional fluorescence-activated cell sorting (FACS) sorter methods. Methods Mol Biol. 2024:2817:33-43. Methods in molecular biology (Clifton, N.J.) 38907145 https://hdl.handle.net/20.500.12105/27057 Mass spectrometry Multiplex sample preparation strategy Orbitrap Eclipse mass spectrometer Single-cell proteomics Tandem mass tag Tribrid mass spectrometer A Sample Preparation Procedure for Isobaric Labeling-Based Single-Cell Proteomics.