2026-05-17T00:18:22Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/270382025-12-18T12:57:33Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Martinez-Laso, Jorge
author
Cervera Hernandez, Isabel
author
Martinez-Carrasco, Marina S
author
Sánchez-Menéndez, Clara
author
Remesal, Manuel
author
Casado-Fernández, Guiomar
author
Mateos, Elena
author
Lemus-Aguilar, Luis
author
Torres, Montserrat
author
Coiras, Mayte
funder
Instituto de Salud Carlos III
funder
Centro de Investigación Biomédica en Red - CIBERINFEC (Enfermedades Infecciosas)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Comunidad de Madrid (España)
funder
Fundación para la Investigación Biomédica del Hospital Universitario Ramón y Cajal
2025-12-15T18:34:19Z
2025-12-15T18:34:19Z
2025-07
Martinez-Laso J, Cervera I, Martinez-Carrasco MS, Sánchez-Menéndez C, Remesal M, Casado-Fernández G, Mateos E, Lemus-Aguilar L, Torres M, Coiras M. Truncated IFI16 mRNA transcripts can control its viral DNA defense activity. Mol Immunol. 2025 Jul;183:137-144. doi: 10.1016/j.molimm.2025.05.005. Epub 2025 May 12. PMID: 40359721.
10.1016/j.molimm.2025.05.005
Molecular Immunology
40359721
https://hdl.handle.net/20.500.12105/27038
One of the most well-known viral receptors of the group called named ALRs is IFI16 (interferon-inducible protein 16) that are responsible for responses against viral dsDNA. A pyrin domain (PYD), two HIN domains, a NLS (nuclear localization sequence), and S/T/P repeats region form the structure of IFI16. Five alternatively transcripts have been described (V1, V2, V9, V4 and Vβ) that encode five isoforms (IFI16-iso1, 2, 3, 4 and β) with different structure, localization, and function. Another four transcripts (V3, V5, V6, and V8) and 12 predicted transcripts (VX1-VX7, VX1.1-VX5.1) have also been registered in the Genebank without any structural study. In the present study, we have performed a complete study of the presence of the IFI16 transcripts in a healthy population. All the alternative transcripts described except six of the so-called predicted transcripts were found, furthermore, two new transcripts (V10, V11) were described. The main mechanisms for the regulation of mRNA from IFI16 expression are due to the insertion of non-coding regions and the loss of almost all exons. A total of nine different isoforms were found and the corresponding protein models were constructed to establish the modification of its functionality to form inflammasomes or the binding to viral DNA.
eng
ALRs
Alternative RNA splicing
IFI16
Isoforms
Truncated IFI16 mRNA transcripts can control its viral DNA defense activity.
research article
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URL
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TruncatedIFI16mRNAtranscripts_2025.pdf