2026-05-17T00:20:31Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/270222025-12-18T13:01:58Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Lozano-Prieto, Marta
author
Jorge, Inmaculada
author
Camafeita, Emilio
author
Devesa, Cristina A
author
Barrero-Rodríguez, Rafael
author
Calvo, Enrique
author
Laguillo-Gómez, Andrea
author
Pertusa, Clara
author
Martin-Cofreces, Noa B
author
Vázquez, Jesús
author
Sánchez-Madrid, Francisco
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2025-12-15T12:47:40Z
2025-12-15T12:47:40Z
2025-12
Eur J Cell Biol. 2025 Dec;104(4):151521.
EUROPEAN JOURNAL OF CELL BIOLOGY
41046742
https://hdl.handle.net/20.500.12105/27022
The shift from quiescent to effector T cells (TC) is controlled at the translational level. Post-translational modifications (PTMs) are key factors in the diversification of protein function. Advancements in mass spectrometry-based proteomics enable proteome-wide, hypothesis-free quantitative PTM analysis. Current research highlights the centrosome role in TC activation. Here the diversity of PTMs in the TC centrosome is studied by analyzing centrosome-enriched fractions from human resting and activated T lymphoblasts. Our results show that oxidative modifications predominate in this organelle, with tryptophan as the most frequently oxidized residue. These PTMs are enriched in proteins involved in translation, vesicular trafficking, cytoskeleton organization, among others. We also demonstrate the existence of PTM changes on specific protein regions during TC activation in Myh9 (hyperoxidized), and Gzma (hypoxidized). These hyper- or hypoxidized proteins display distinct functional distributions. Our study provides the first comprehensive PTM mapping of the TC centrosome, underscoring the PTM regulatory role in TC activation.
eng
Centrosome
Post-translational modifications
Proteomics
T lymphocytes
Deciphering the landscape of post-translational modifications in the centrosome upon T cell activation.
research article
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URL
https://repisalud.isciii.es/bitstreams/a2db768b-89c6-4533-92c7-a662f41257bd/download
File
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b144d29875b22dcb11bfd3d66234046e
5478076
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Deciphering the landscape of post-translational_Eur J Cell Biol_2025.pdf