2026-05-17T00:20:31Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/270222025-12-18T13:01:58Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Lozano-Prieto, Marta author Jorge, Inmaculada author Camafeita, Emilio author Devesa, Cristina A author Barrero-Rodríguez, Rafael author Calvo, Enrique author Laguillo-Gómez, Andrea author Pertusa, Clara author Martin-Cofreces, Noa B author Vázquez, Jesús author Sánchez-Madrid, Francisco author 2025-12 The shift from quiescent to effector T cells (TC) is controlled at the translational level. Post-translational modifications (PTMs) are key factors in the diversification of protein function. Advancements in mass spectrometry-based proteomics enable proteome-wide, hypothesis-free quantitative PTM analysis. Current research highlights the centrosome role in TC activation. Here the diversity of PTMs in the TC centrosome is studied by analyzing centrosome-enriched fractions from human resting and activated T lymphoblasts. Our results show that oxidative modifications predominate in this organelle, with tryptophan as the most frequently oxidized residue. These PTMs are enriched in proteins involved in translation, vesicular trafficking, cytoskeleton organization, among others. We also demonstrate the existence of PTM changes on specific protein regions during TC activation in Myh9 (hyperoxidized), and Gzma (hypoxidized). These hyper- or hypoxidized proteins display distinct functional distributions. Our study provides the first comprehensive PTM mapping of the TC centrosome, underscoring the PTM regulatory role in TC activation. Eur J Cell Biol. 2025 Dec;104(4):151521. EUROPEAN JOURNAL OF CELL BIOLOGY 41046742 https://hdl.handle.net/20.500.12105/27022 Centrosome Post-translational modifications Proteomics T lymphocytes Deciphering the landscape of post-translational modifications in the centrosome upon T cell activation.