2026-05-17T00:18:34Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/269922025-12-18T13:01:57Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Zamora-Dorta, Marcos
author
Laine-Menéndez, Sara
author
Abia, David
author
González-García, Pilar
author
López, Luis C
author
Fernández-Montes, Paula
author
Calvo, Enrique
author
Vázquez, Jesús
author
Enríquez, José Antonio
author
Balsa, Eduardo
2025-12-09T16:09:07Z
2025-12-09T16:09:07Z
2025-06
EMBO Rep. 2025 Jun;26(12):3045-3074.
40301572
https://hdl.handle.net/20.500.12105/26992
Defects in mitochondrial oxidative metabolism underlie many genetic disorders with limited treatment options. The incomplete annotation of mitochondrial proteins highlights the need for a comprehensive gene inventory, particularly for Oxidative Phosphorylation (OXPHOS). To address this, we developed a CRISPR/Cas9 loss-of-function library targeting nuclear-encoded mitochondrial genes and conducted galactose-based screenings to identify novel regulators of mitochondrial function. Our study generates a gene catalog essential for mitochondrial metabolism and maps a dynamic network of mitochondrial pathways, focusing on OXPHOS complexes. Computational analysis identifies RTN4IP1 and ECHS1 as key OXPHOS genes linked to mitochondrial diseases in humans. RTN4IP1 is found to be crucial for mitochondrial respiration, with complexome profiling revealing its role as an assembly factor required for the complete assembly of complex I. Furthermore, we discovered that ECHS1 controls oxidative metabolism independently of its canonical function in fatty acid oxidation. Its deletion impairs branched-chain amino acids (BCAA) catabolism, disrupting lipoic acid-dependent enzymes such as pyruvate dehydrogenase (PDH). This deleterious phenotype can be rescued by restricting valine intake or catabolism in ECHS1-deficient cells.
eng
CRISPR Screening
ECHS1
Mitochondria
OXPHOS
RTN4IP1
Time-resolved mitochondrial screen identifies regulatory components of oxidative metabolism.
research article
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URL
https://repisalud.isciii.es/bitstreams/8b5ac3a0-5a16-4cf9-849e-f085815aa510/download
File
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70ee31abab15b82c6df5cf77c0d7a127
6960871
application/pdf
Time-resolved mitochondrial_EMBO Rep_2025.pdf